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Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts.

Publication ,  Journal Article
Lagoo, AS; Buckley, PJ; Burchell, LJ; Peters, D; Fechner, JH; Tsuchida, M; Dong, Y; Hong, X; Brunner, KG; Oberley, TD; Hamawy, MM; Knechtle, SJ
Published in: Transplantation
September 27, 2000

BACKGROUND: In our previously described primate renal allograft model, T cell ablation leads to long-term graft survival. The role of endothelial cell alteration in chronic rejection was examined in our model. METHODS: Renal transplants were performed in rhesus monkeys using a T cell- depleting immunotoxin, FN18-CRM9. Sections from 10 rejected kidneys (5 acute and 7 chronic rejection) were examined after immunohistochemical staining for expression of endothelium-related proteins [von Willebrand factor (vWF), CD62P, and CD31], fibrinogen, and a macrophage marker (CD68). Glomerular staining for each antigen was graded on a semiquantitative scale. RESULTS: Intense staining for vWF was consistently observed in glomerular endothelium, subendothelium, and mesangium in all kidneys removed due to chronic rejection. vWF staining was weak in kidneys showing acute rejection. The difference in glomerular staining was statistically significant. Staining for vWF in extraglomerular vessels was nearly identical in kidneys showing acute and chronic rejection. Expression of CD62P was increased in extraglomerular vessels in allografts with chronic rejection, but the glomeruli showed little or no staining. There was no significant difference in the glomerular staining for CD62P or CD31 in organs showing acute and chronic rejection. Fibrinogen staining of glomerular mesangium was seen in kidneys with chronic rejection. Macrophages (CD68+) infiltrating glomeruli were more numerous in kidneys showing chronic rejection. CONCLUSION: Increased glomerular deposition of vWF in renal allografts showing chronic rejection, without increased staining for CD62P or CD31, suggests increased constitutive secretion of vWF from endothelial cells as a component of the mechanism of chronic rejection in our model.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

September 27, 2000

Volume

70

Issue

6

Start / End Page

877 / 886

Location

United States

Related Subject Headings

  • von Willebrand Factor
  • Surgery
  • Male
  • Macrophages
  • Macaca mulatta
  • Kidney Transplantation
  • Kidney Glomerulus
  • Immunotoxins
  • Immune Tolerance
  • Graft Rejection
 

Citation

APA
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MLA
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Lagoo, A. S., Buckley, P. J., Burchell, L. J., Peters, D., Fechner, J. H., Tsuchida, M., … Knechtle, S. J. (2000). Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation, 70(6), 877–886. https://doi.org/10.1097/00007890-200009270-00005
Lagoo, A. S., P. J. Buckley, L. J. Burchell, D. Peters, J. H. Fechner, M. Tsuchida, Y. Dong, et al. “Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts.Transplantation 70, no. 6 (September 27, 2000): 877–86. https://doi.org/10.1097/00007890-200009270-00005.
Lagoo AS, Buckley PJ, Burchell LJ, Peters D, Fechner JH, Tsuchida M, et al. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000 Sep 27;70(6):877–86.
Lagoo, A. S., et al. “Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts.Transplantation, vol. 70, no. 6, Sept. 2000, pp. 877–86. Pubmed, doi:10.1097/00007890-200009270-00005.
Lagoo AS, Buckley PJ, Burchell LJ, Peters D, Fechner JH, Tsuchida M, Dong Y, Hong X, Brunner KG, Oberley TD, Hamawy MM, Knechtle SJ. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000 Sep 27;70(6):877–886.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

September 27, 2000

Volume

70

Issue

6

Start / End Page

877 / 886

Location

United States

Related Subject Headings

  • von Willebrand Factor
  • Surgery
  • Male
  • Macrophages
  • Macaca mulatta
  • Kidney Transplantation
  • Kidney Glomerulus
  • Immunotoxins
  • Immune Tolerance
  • Graft Rejection