The coenrichment of stem cells, prothymocytes, and stromal elements with ecotropic retrovirus-producing cells from the bone marrow of leukemia-prone AKR mice.
Ecotropic virus-producing cells in the bone marrow of the leukemia-prone AKR strain of mice were significantly enriched by fractionation on discontinuous density gradients of Percoll and were found in a low-density population of cells comprised predominantly of medium to large blast cells. The high ecotropic virus-producing low-density bone marrow cell population was also found to be significantly enriched in pluripotent stem cells, prothymocytes, and stromal elements. During the period of time defined by a window for successful leukemosuppressive immunotherapy of AKR mice, virus-producing cells were exclusively detected in this fraction of bone marrow cells, implicating the functional classes of cells coenriched in this fraction as both potential targets of anti-viral immunotherapy and responsible for the seeding of the spleen and thymus with infectious ecotropic virus.
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Related Subject Headings
- Virology
- Viral Proteins
- Thymus Gland
- Stem Cells
- Phenotype
- Mice, Inbred AKR
- Mice
- Leukemia Virus, Murine
- Cell Separation
- Bone Marrow Cells
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Virology
- Viral Proteins
- Thymus Gland
- Stem Cells
- Phenotype
- Mice, Inbred AKR
- Mice
- Leukemia Virus, Murine
- Cell Separation
- Bone Marrow Cells