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Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2.

Publication ,  Journal Article
White, RR; Shan, S; Rusconi, CP; Shetty, G; Dewhirst, MW; Kontos, CD; Sullenger, BA
Published in: Proc Natl Acad Sci U S A
April 29, 2003

Angiopoietin-2 (Ang2) appears to be a naturally occurring antagonist of the endothelial receptor tyrosine kinase Tie2, an important regulator of vascular stability. Destabilization of the endothelium by Ang2 is believed to potentiate the actions of proangiogenic growth factors. To investigate the specific role of Ang2 in the adult vasculature, we generated a nuclease-resistant RNA aptamer that binds and inhibits Ang2 but not the related Tie2 agonist, angiopoietin-1. Local delivery of this aptamer but not a partially scrambled mutant aptamer inhibited basic fibroblast growth factor-mediated neovascularization in the rat corneal micropocket angiogenesis assay. These in vivo data directly demonstrate that a specific inhibitor of Ang2 can act as an antiangiogenic agent.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 29, 2003

Volume

100

Issue

9

Start / End Page

5028 / 5033

Location

United States

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • RNA
  • Protein Binding
  • Precipitin Tests
  • Neovascularization, Pathologic
  • Humans
  • Female
  • DNA Primers
  • Cornea
 

Citation

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White, R. R., Shan, S., Rusconi, C. P., Shetty, G., Dewhirst, M. W., Kontos, C. D., & Sullenger, B. A. (2003). Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2. Proc Natl Acad Sci U S A, 100(9), 5028–5033. https://doi.org/10.1073/pnas.0831159100
White, Rebekah R., Siqing Shan, Christopher P. Rusconi, Geetha Shetty, Mark W. Dewhirst, Christopher D. Kontos, and Bruce A. Sullenger. “Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2.Proc Natl Acad Sci U S A 100, no. 9 (April 29, 2003): 5028–33. https://doi.org/10.1073/pnas.0831159100.
White RR, Shan S, Rusconi CP, Shetty G, Dewhirst MW, Kontos CD, et al. Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5028–33.
White, Rebekah R., et al. “Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2.Proc Natl Acad Sci U S A, vol. 100, no. 9, Apr. 2003, pp. 5028–33. Pubmed, doi:10.1073/pnas.0831159100.
White RR, Shan S, Rusconi CP, Shetty G, Dewhirst MW, Kontos CD, Sullenger BA. Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5028–5033.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 29, 2003

Volume

100

Issue

9

Start / End Page

5028 / 5033

Location

United States

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • RNA
  • Protein Binding
  • Precipitin Tests
  • Neovascularization, Pathologic
  • Humans
  • Female
  • DNA Primers
  • Cornea