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Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice.

Publication ,  Journal Article
Sarraf-Yazdi, S; Mi, J; Dewhirst, MW; Clary, BM
Published in: Eur J Surg Oncol
April 2008

BACKGROUND: The impact of partial hepatectomy on intra-hepatic and distant tumor growth is a matter of controversy. Utilizing a highly sensitive tumor imaging strategy, we sought to demonstrate whether this growth-acceleration occurs, and to develop an animal model with which to investigate potential therapeutic strategies. METHODS: Mice bearing constitutively-active luciferase-expressing tumor cells were subjected to either 70% partial hepatectomy (PH; n=10) or a sham operation (n=11). Mice were sacrificed 14 days later and remnant livers (or anatomic equivalents in the control group) and lungs harvested for bioluminescence detection. RESULTS: Remnant liver weights were significantly increased in PH compared to equivalent lobes in sham-operated animals (t-test; p=0.005). Tumor burden as measured by bioluminescence was significantly higher in both liver and lung specimens in the PH group (Wilcoxon's Rank Sum test; p=0.01 and 0.004, respectively). CONCLUSIONS: Following PH, enhanced metastatic growth was depicted regionally and systemically with bioluminescence imaging providing an objective measure of tumor burden. This preclinical model can help to identify adjuvant therapies that can influence both tumor growth and liver regeneration.

Duke Scholars

Published In

Eur J Surg Oncol

DOI

EISSN

1532-2157

Publication Date

April 2008

Volume

34

Issue

4

Start / End Page

476 / 481

Location

England

Related Subject Headings

  • Tumor Burden
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Mice, Inbred BALB C
  • Mice
  • Lung Neoplasms
  • Luminescent Agents
  • Luciferases
  • Liver Neoplasms
  • Hepatectomy
 

Citation

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ICMJE
MLA
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Sarraf-Yazdi, S., Mi, J., Dewhirst, M. W., & Clary, B. M. (2008). Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice. Eur J Surg Oncol, 34(4), 476–481. https://doi.org/10.1016/j.ejso.2007.06.001
Sarraf-Yazdi, S., J. Mi, M. W. Dewhirst, and B. M. Clary. “Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice.Eur J Surg Oncol 34, no. 4 (April 2008): 476–81. https://doi.org/10.1016/j.ejso.2007.06.001.
Sarraf-Yazdi S, Mi J, Dewhirst MW, Clary BM. Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice. Eur J Surg Oncol. 2008 Apr;34(4):476–81.
Sarraf-Yazdi, S., et al. “Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice.Eur J Surg Oncol, vol. 34, no. 4, Apr. 2008, pp. 476–81. Pubmed, doi:10.1016/j.ejso.2007.06.001.
Sarraf-Yazdi S, Mi J, Dewhirst MW, Clary BM. Use of bioluminescence imaging to detect enhanced hepatic and systemic tumor growth following partial hepatectomy in mice. Eur J Surg Oncol. 2008 Apr;34(4):476–481.
Journal cover image

Published In

Eur J Surg Oncol

DOI

EISSN

1532-2157

Publication Date

April 2008

Volume

34

Issue

4

Start / End Page

476 / 481

Location

England

Related Subject Headings

  • Tumor Burden
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Mice, Inbred BALB C
  • Mice
  • Lung Neoplasms
  • Luminescent Agents
  • Luciferases
  • Liver Neoplasms
  • Hepatectomy