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The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints.

Publication ,  Journal Article
Fowler, VG; Fey, PD; Reller, LB; Chamis, AL; Corey, GR; Rupp, ME
Published in: Med Microbiol Immunol
April 2001

Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

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Published In

Med Microbiol Immunol

DOI

ISSN

0300-8584

Publication Date

April 2001

Volume

189

Issue

3

Start / End Page

127 / 131

Location

Germany

Related Subject Headings

  • Staphylococcus aureus
  • Staphylococcal Infections
  • Prospective Studies
  • Polysaccharides, Bacterial
  • N-Acetylglucosaminyltransferases
  • Middle Aged
  • Male
  • Joint Prosthesis
  • Intracellular Fluid
  • Humans
 

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Fowler, V. G., Fey, P. D., Reller, L. B., Chamis, A. L., Corey, G. R., & Rupp, M. E. (2001). The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints. Med Microbiol Immunol, 189(3), 127–131. https://doi.org/10.1007/s430-001-8018-5
Fowler, V. G., P. D. Fey, L. B. Reller, A. L. Chamis, G. R. Corey, and M. E. Rupp. “The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints.Med Microbiol Immunol 189, no. 3 (April 2001): 127–31. https://doi.org/10.1007/s430-001-8018-5.
Fowler, V. G., et al. “The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints.Med Microbiol Immunol, vol. 189, no. 3, Apr. 2001, pp. 127–31. Pubmed, doi:10.1007/s430-001-8018-5.
Fowler VG, Fey PD, Reller LB, Chamis AL, Corey GR, Rupp ME. The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints. Med Microbiol Immunol. 2001 Apr;189(3):127–131.
Journal cover image

Published In

Med Microbiol Immunol

DOI

ISSN

0300-8584

Publication Date

April 2001

Volume

189

Issue

3

Start / End Page

127 / 131

Location

Germany

Related Subject Headings

  • Staphylococcus aureus
  • Staphylococcal Infections
  • Prospective Studies
  • Polysaccharides, Bacterial
  • N-Acetylglucosaminyltransferases
  • Middle Aged
  • Male
  • Joint Prosthesis
  • Intracellular Fluid
  • Humans