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Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos.

Publication ,  Journal Article
Coin, PG; Osornio-Vargas, AR; Roggli, VL; Brody, AR
Published in: Am J Respir Crit Care Med
November 1996

Previously, this laboratory developed a model of asbestos-induced pulmonary fibrogenesis in rats and mice after a brief (1 to 3-h) inhalation exposure. However, typical human environmental exposures would be repeated, although at lower concentrations than those used in our animal model. Here we have extended this model to encompass repeated exposures and consequent long-term effects. Groups of rats were exposed to chrysotile aerosol (10 mg/m3) for 3- to 5-h periods over 3 consecutive days. Lung fiber burden and pathologic features were studied for as long as 6 mo after exposure. We found that many of the longest (> or = 8 microm) fibers were retained in the lung for at least 6 mo, whereas shorter fibers were cleared more rapidly. The three exposures to chrysotile caused a large increase in DNA synthesis in the epithelium of terminal bronchioles and more proximal airways. When compared with a single exposure, the triple exposure caused an enhanced inflammatory response as well as a prolonged period of increased DNA synthesis in the proximal alveolar region. Hyperplastic, fibrotic lesions subsequently developed in the same region and persisted for at least 6 mo after exposure. These findings will be valuable in directing future studies of the mechanisms of pulmonary fibrosis in this model.

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Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

November 1996

Volume

154

Issue

5

Start / End Page

1511 / 1519

Location

United States

Related Subject Headings

  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Pulmonary Fibrosis
  • Male
  • Macrophages, Alveolar
  • Dose-Response Relationship, Drug
  • DNA
  • Asbestos, Serpentine
  • Animals
 

Citation

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Coin, P. G., Osornio-Vargas, A. R., Roggli, V. L., & Brody, A. R. (1996). Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos. Am J Respir Crit Care Med, 154(5), 1511–1519. https://doi.org/10.1164/ajrccm.154.5.8912773
Coin, P. G., A. R. Osornio-Vargas, V. L. Roggli, and A. R. Brody. “Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos.Am J Respir Crit Care Med 154, no. 5 (November 1996): 1511–19. https://doi.org/10.1164/ajrccm.154.5.8912773.
Coin PG, Osornio-Vargas AR, Roggli VL, Brody AR. Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos. Am J Respir Crit Care Med. 1996 Nov;154(5):1511–9.
Coin, P. G., et al. “Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos.Am J Respir Crit Care Med, vol. 154, no. 5, Nov. 1996, pp. 1511–19. Pubmed, doi:10.1164/ajrccm.154.5.8912773.
Coin PG, Osornio-Vargas AR, Roggli VL, Brody AR. Pulmonary fibrogenesis after three consecutive inhalation exposures to chrysotile asbestos. Am J Respir Crit Care Med. 1996 Nov;154(5):1511–1519.

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

November 1996

Volume

154

Issue

5

Start / End Page

1511 / 1519

Location

United States

Related Subject Headings

  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Pulmonary Fibrosis
  • Male
  • Macrophages, Alveolar
  • Dose-Response Relationship, Drug
  • DNA
  • Asbestos, Serpentine
  • Animals