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Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis.

Publication ,  Journal Article
Potts, SB; Roggli, VL; Spock, A
Published in: Pediatr Pathol Lab Med
1995

Pseudomonas aeruginosa has been recognized as a pathogen of major importance in the patient with cystic fibrosis (CF). However, no information is available regarding the histologic quantification of P. aeruginosa organisms in the CF tracheobronchial tree. We retrieved all formalin-fixed paraffin-embedded lung blocks from 20 consecutive autopsies of cystic fibrosis patients. Serial histologic sections were made and stained by three methods: hematoxylin and eosin, immunoperoxidase with anti-P. aeruginosa rabbit serum as the primary antibody, and immunoperoxidase with normal rabbit serum as the primary antibody. By studying the hematoxylin and eosin section, we classified five areas in the lung as bronchi, large bronchioles, small bronchioles, bronchioloectatic areas, and abscess/airways with destroyed epithelium. The areas stained by an anti-P. aeruginosa immunoperoxidase method were examined under high-power magnification, and the bacteria within random fields were counted. Pseudomonas aeruginosa organisms were identified in 14 of 20 cases, including 13 of 16 cases in which P. aeruginosa was specifically cultured at autopsy. Quantification of organisms within the lumens of all five airway types showed that the bacterial density in cystic fibrosis airways is highest in bronchi.

Duke Scholars

Published In

Pediatr Pathol Lab Med

DOI

ISSN

1077-1042

Publication Date

1995

Volume

15

Issue

5

Start / End Page

707 / 721

Location

United States

Related Subject Headings

  • Trachea
  • Staining and Labeling
  • Rabbits
  • Pseudomonas aeruginosa
  • Pseudomonas Infections
  • Male
  • Infant
  • Humans
  • Female
  • Cystic Fibrosis
 

Citation

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ICMJE
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Potts, S. B., Roggli, V. L., & Spock, A. (1995). Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis. Pediatr Pathol Lab Med, 15(5), 707–721. https://doi.org/10.3109/15513819509027007
Potts, S. B., V. L. Roggli, and A. Spock. “Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis.Pediatr Pathol Lab Med 15, no. 5 (1995): 707–21. https://doi.org/10.3109/15513819509027007.
Potts, S. B., et al. “Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis.Pediatr Pathol Lab Med, vol. 15, no. 5, 1995, pp. 707–21. Pubmed, doi:10.3109/15513819509027007.

Published In

Pediatr Pathol Lab Med

DOI

ISSN

1077-1042

Publication Date

1995

Volume

15

Issue

5

Start / End Page

707 / 721

Location

United States

Related Subject Headings

  • Trachea
  • Staining and Labeling
  • Rabbits
  • Pseudomonas aeruginosa
  • Pseudomonas Infections
  • Male
  • Infant
  • Humans
  • Female
  • Cystic Fibrosis