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Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex.

Publication ,  Journal Article
Xiao, K; Engstrom, G; Rajagukguk, S; Yu, C-A; Yu, L; Durham, B; Millett, F
Published in: J Biol Chem
March 28, 2003

Famoxadone is a new cytochrome bc(1) Q(o) site inhibitor that immobilizes the iron-sulfur protein (ISP) in the b conformation. The effects of famoxadone on electron transfer between the iron-sulfur center (2Fe-2S) and cyt c(1) were studied using a ruthenium dimer to photoinitiate the reaction. The rate constant for electron transfer in the forward direction from 2Fe-2S to cyt c(1) was found to be 16,000 s(-1) in bovine cyt bc(1). Binding famoxadone decreased this rate constant to 1,480 s(-1), consistent with a decrease in mobility of the ISP. Reverse electron transfer from cyt c(1) to 2Fe-2S was found to be biphasic in bovine cyt bc(1) with rate constants of 90,000 and 7,300 s(-1). In the presence of famoxadone, reverse electron transfer was monophasic with a rate constant of 1,420 s(-1). It appears that the rate constants for the release of the oxidized and reduced ISP from the b conformation are the same in the presence of famoxadone. The effects of famoxadone binding on electron transfer were also studied in a series of Rhodobacter sphaeroides cyt bc(1) mutants involving residues at the interface between the Rieske protein and cyt c(1) and/or cyt b.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 28, 2003

Volume

278

Issue

13

Start / End Page

11419 / 11426

Location

United States

Related Subject Headings

  • Strobilurins
  • Pyrimidines
  • Protein Conformation
  • Photochemistry
  • Oxazoles
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Methacrylates
  • Kinetics
  • Iron-Sulfur Proteins
 

Citation

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Xiao, K., Engstrom, G., Rajagukguk, S., Yu, C.-A., Yu, L., Durham, B., & Millett, F. (2003). Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex. J Biol Chem, 278(13), 11419–11426. https://doi.org/10.1074/jbc.M211620200
Xiao, Kunhong, Gregory Engstrom, Sany Rajagukguk, Chang-An Yu, Linda Yu, Bill Durham, and Francis Millett. “Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex.J Biol Chem 278, no. 13 (March 28, 2003): 11419–26. https://doi.org/10.1074/jbc.M211620200.
Xiao K, Engstrom G, Rajagukguk S, Yu C-A, Yu L, Durham B, et al. Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex. J Biol Chem. 2003 Mar 28;278(13):11419–26.
Xiao, Kunhong, et al. “Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex.J Biol Chem, vol. 278, no. 13, Mar. 2003, pp. 11419–26. Pubmed, doi:10.1074/jbc.M211620200.
Xiao K, Engstrom G, Rajagukguk S, Yu C-A, Yu L, Durham B, Millett F. Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex. J Biol Chem. 2003 Mar 28;278(13):11419–11426.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 28, 2003

Volume

278

Issue

13

Start / End Page

11419 / 11426

Location

United States

Related Subject Headings

  • Strobilurins
  • Pyrimidines
  • Protein Conformation
  • Photochemistry
  • Oxazoles
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Methacrylates
  • Kinetics
  • Iron-Sulfur Proteins