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In vivo identification of regulators of cell invasion across basement membranes.

Publication ,  Journal Article
Matus, DQ; Li, XY; Durbin, S; Agarwal, D; Chi, Q; Weiss, SJ; Sherwood, DR
Published in: Science signaling
2010

Cell invasion through basement membranes during development, immune surveillance, and metastasis remains poorly understood. To gain further insight into this key cellular behavior, we performed an in vivo screen for regulators of cell invasion through basement membranes, using the simple model of Caenorhabditis elegans anchor cell invasion, and identified 99 genes that promote invasion, including the genes encoding the chaperonin complex cct. Notably, most of these genes have not been previously implicated in invasive cell behavior. We characterized members of the cct complex and 11 other gene products, determining the distinct aspects of the invasive cascade that they regulate, including formation of a specialized invasive cell membrane and its ability to breach the basement membrane. RNA interference-mediated knockdown of the human orthologs of cct-5 and lit-1, which had not previously been implicated in cell invasion, reduced the invasiveness of metastatic carcinoma cells, suggesting that a conserved genetic program underlies cell invasion. These results increase our understanding of the genetic underpinnings of cell invasion and also provide new potential therapeutic targets to limit this behavior.

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Published In

Science signaling

DOI

ISSN

1937-9145

Publication Date

2010

Volume

3

Issue

120

Start / End Page

ra35

Related Subject Headings

  • RNA Interference
  • Neoplasm Invasiveness
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

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Matus, D. Q., Li, X. Y., Durbin, S., Agarwal, D., Chi, Q., Weiss, S. J., & Sherwood, D. R. (2010). In vivo identification of regulators of cell invasion across basement membranes. Science Signaling, 3(120), ra35. https://doi.org/10.1126/scisignal.2000654
Matus, D. Q., X. Y. Li, S. Durbin, D. Agarwal, Q. Chi, S. J. Weiss, and D. R. Sherwood. “In vivo identification of regulators of cell invasion across basement membranes.Science Signaling 3, no. 120 (2010): ra35. https://doi.org/10.1126/scisignal.2000654.
Matus DQ, Li XY, Durbin S, Agarwal D, Chi Q, Weiss SJ, et al. In vivo identification of regulators of cell invasion across basement membranes. Science signaling. 2010;3(120):ra35.
Matus, D. Q., et al. “In vivo identification of regulators of cell invasion across basement membranes.Science Signaling, vol. 3, no. 120, 2010, p. ra35. Manual, doi:10.1126/scisignal.2000654.
Matus DQ, Li XY, Durbin S, Agarwal D, Chi Q, Weiss SJ, Sherwood DR. In vivo identification of regulators of cell invasion across basement membranes. Science signaling. 2010;3(120):ra35.

Published In

Science signaling

DOI

ISSN

1937-9145

Publication Date

2010

Volume

3

Issue

120

Start / End Page

ra35

Related Subject Headings

  • RNA Interference
  • Neoplasm Invasiveness
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology