Mapping Drosophila genomic aberration breakpoints with comparative genome hybridization on microarrays.
Chromosomal aberrations are genetic "reagents" that are commonly used in Drosophila research. Stocks containing chromosomes carrying large deletions of DNA (deficiency stocks, designated Df) as well as stocks carrying an extra copy of a chromosomal region (duplication stocks, designated Dp) are essential for a variety of genetic analyses. The extent of what is deleted or duplicated has typically been determined cytologically by salivary gland polytene chromosome squashes, which identify the edges of the aberration (so-called breakpoints) of each Df or Dp at low resolution. The margin of error for this technique can be quite high, however, because it is dependent on the quality of the squash and the experience of the scientist interpreting the data. Comparative genome hybridization on microarrays provides a precise molecular method to identify which regions of the genome are deleted or duplicated in these stocks by examining a change in chromosomal ploidy across the whole genome. Furthermore, this technique allows genetic data obtained with these strains to be placed in a molecular genomic context.
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Related Subject Headings
- Oligonucleotide Array Sequence Analysis
- Nucleic Acid Hybridization
- Genome, Insect
- Drosophila
- Chromosome Mapping
- Chromosome Breakage
- Biochemistry & Molecular Biology
- Animals
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Start / End Page
Related Subject Headings
- Oligonucleotide Array Sequence Analysis
- Nucleic Acid Hybridization
- Genome, Insect
- Drosophila
- Chromosome Mapping
- Chromosome Breakage
- Biochemistry & Molecular Biology
- Animals
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology