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Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1.

Publication ,  Journal Article
McKay, JK; Richards, JH; Nemali, KS; Sen, S; Mitchell-Olds, T; Boles, S; Stahl, EA; Wayne, T; Juenger, TE
Published in: Evolution; international journal of organic evolution
December 2008

Despite compelling evidence that adaptation to local climate is common in plant populations, little is known about the evolutionary genetics of traits that contribute to climatic adaptation. A screen of natural accessions of Arabidopsis thaliana revealed Tsu-1 and Kas-1 to be opposite extremes for water-use efficiency and climate at collection sites for these accessions differs greatly. To provide a tool to understand the genetic basis of this putative adaptation, Kas-1 and Tsu-1 were reciprocally crossed to create a new mapping population. Analysis of F(3) families showed segregating variation in both delta(13)C and transpiration rate, and as expected these traits had a negative genetic correlation (r(g)=- 0.3). 346 RILs, 148 with Kas-1 cytoplasm and 198 with Tsu-1 cytoplasm, were advanced to the F(9) and genotyped using 48 microsatellites and 55 SNPs for a total of 103 markers. This mapping population was used for QTL analysis of delta(13)C using F(9) RIL means. Analysis of this reciprocal cross showed a large effect of cytoplasmic background, as well as two QTL for delta(13)C. The Kas-1 x Tsu-1 mapping population provides a powerful new resource for mapping QTL underlying natural variation and for dissecting the genetic basis of water-use efficiency differences.

Duke Scholars

Published In

Evolution; international journal of organic evolution

DOI

EISSN

1558-5646

ISSN

0014-3820

Publication Date

December 2008

Volume

62

Issue

12

Start / End Page

3014 / 3026

Related Subject Headings

  • Species Specificity
  • Quantitative Trait Loci
  • Polymorphism, Single Nucleotide
  • Microsatellite Repeats
  • Genotype
  • Genetic Markers
  • Evolutionary Biology
  • Droughts
  • Crosses, Genetic
  • Arabidopsis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McKay, J. K., Richards, J. H., Nemali, K. S., Sen, S., Mitchell-Olds, T., Boles, S., … Juenger, T. E. (2008). Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1. Evolution; International Journal of Organic Evolution, 62(12), 3014–3026. https://doi.org/10.1111/j.1558-5646.2008.00474.x
McKay, John K., James H. Richards, Krishna S. Nemali, Saunak Sen, Thomas Mitchell-Olds, Sandra Boles, Eli A. Stahl, Tierney Wayne, and Thomas E. Juenger. “Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1.Evolution; International Journal of Organic Evolution 62, no. 12 (December 2008): 3014–26. https://doi.org/10.1111/j.1558-5646.2008.00474.x.
McKay JK, Richards JH, Nemali KS, Sen S, Mitchell-Olds T, Boles S, et al. Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1. Evolution; international journal of organic evolution. 2008 Dec;62(12):3014–26.
McKay, John K., et al. “Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1.Evolution; International Journal of Organic Evolution, vol. 62, no. 12, Dec. 2008, pp. 3014–26. Epmc, doi:10.1111/j.1558-5646.2008.00474.x.
McKay JK, Richards JH, Nemali KS, Sen S, Mitchell-Olds T, Boles S, Stahl EA, Wayne T, Juenger TE. Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1. Evolution; international journal of organic evolution. 2008 Dec;62(12):3014–3026.
Journal cover image

Published In

Evolution; international journal of organic evolution

DOI

EISSN

1558-5646

ISSN

0014-3820

Publication Date

December 2008

Volume

62

Issue

12

Start / End Page

3014 / 3026

Related Subject Headings

  • Species Specificity
  • Quantitative Trait Loci
  • Polymorphism, Single Nucleotide
  • Microsatellite Repeats
  • Genotype
  • Genetic Markers
  • Evolutionary Biology
  • Droughts
  • Crosses, Genetic
  • Arabidopsis