Skip to main content
Journal cover image

Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses.

Publication ,  Journal Article
Wang, S; Durrant, WE; Song, J; Spivey, NW; Dong, X
Published in: Proceedings of the National Academy of Sciences of the United States of America
December 2010

Systemic acquired resistance (SAR) is a plant immune response associated with both transcriptional reprogramming and increased homologous DNA recombination (HR). SNI1 is a negative regulator of SAR and HR, as indicated by the increased basal expression of defense genes and HR in sni1. We found that the sni1 phenotypes are rescued by mutations in BREAST CANCER 2 (BRCA2). In humans, BRCA2 is a mediator of RAD51 in pairing of homologous DNA. Mutations in BRCA2 cause predisposition to breast/ovarian cancers; however, the role of the BRCA2-RAD51 complex in transcriptional regulation remains unclear. In Arabidopsis, both brca2 and rad51 were found to be hypersusceptible not only to genotoxic substances, but also to pathogen infections. A whole-genome microarray analysis showed that downstream of NPR1, BRCA2A is a major regulator of defense-related gene transcription. ChIP demonstrated that RAD51 is specifically recruited to the promoters of defense genes during SAR. This recruitment is dependent on the SAR signal salicylic acid (SA) and on the function of BRCA2. This study provides the molecular evidence showing that the BRCA2-RAD51 complex, known for its function in HR, also plays a direct and specific role in transcription regulation during plant immune responses.

Duke Scholars

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

December 2010

Volume

107

Issue

52

Start / End Page

22716 / 22721

Related Subject Headings

  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Salicylates
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rad51 Recombinase
  • Pseudomonas syringae
  • Protein Binding
  • Plant Diseases
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Synthesis Inhibitors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, S., Durrant, W. E., Song, J., Spivey, N. W., & Dong, X. (2010). Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses. Proceedings of the National Academy of Sciences of the United States of America, 107(52), 22716–22721. https://doi.org/10.1073/pnas.1005978107
Wang, Shui, Wendy E. Durrant, Junqi Song, Natalie W. Spivey, and Xinnian Dong. “Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses.Proceedings of the National Academy of Sciences of the United States of America 107, no. 52 (December 2010): 22716–21. https://doi.org/10.1073/pnas.1005978107.
Wang S, Durrant WE, Song J, Spivey NW, Dong X. Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses. Proceedings of the National Academy of Sciences of the United States of America. 2010 Dec;107(52):22716–21.
Wang, Shui, et al. “Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses.Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 52, Dec. 2010, pp. 22716–21. Epmc, doi:10.1073/pnas.1005978107.
Wang S, Durrant WE, Song J, Spivey NW, Dong X. Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses. Proceedings of the National Academy of Sciences of the United States of America. 2010 Dec;107(52):22716–22721.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

December 2010

Volume

107

Issue

52

Start / End Page

22716 / 22721

Related Subject Headings

  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Salicylates
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rad51 Recombinase
  • Pseudomonas syringae
  • Protein Binding
  • Plant Diseases
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Synthesis Inhibitors