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A comprehensive method for optical-emission computed tomography.

Publication ,  Journal Article
Thomas, A; Bowsher, J; Roper, J; Oliver, T; Dewhirst, M; Oldham, M
Published in: Phys Med Biol
July 21, 2010

Optical-computed tomography (CT) and optical-emission computed tomography (ECT) are recent techniques with potential for high-resolution multi-faceted 3D imaging of the structure and function in unsectioned tissue samples up to 1-4 cc. Quantitative imaging of 3D fluorophore distribution (e.g. GFP) using optical-ECT is challenging due to attenuation present within the sample. Uncorrected reconstructed images appear hotter near the edges than at the center. A similar effect is seen in SPECT/PET imaging, although an important difference is attenuation occurs for both emission and excitation photons. This work presents a way to implement not only the emission attenuation correction utilized in SPECT, but also excitation attenuation correction and source strength modeling which are unique to optical-ECT. The performance of the correction methods was investigated by the use of a cylindrical gelatin phantom whose central region was filled with a known distribution of attenuation and fluorophores. Uncorrected and corrected reconstructions were compared to a sectioned slice of the phantom imaged using a fluorescent dissecting microscope. Significant attenuation artifacts were observed in uncorrected images and appeared up to 80% less intense in the central regions due to attenuation and an assumed uniform light source. The corrected reconstruction showed agreement throughout the verification image with only slight variations ( approximately 5%). Final experiments demonstrate the correction in tissue as applied to a tumor with constitutive RFP.

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Published In

Phys Med Biol

DOI

EISSN

1361-6560

Publication Date

July 21, 2010

Volume

55

Issue

14

Start / End Page

3947 / 3957

Location

England

Related Subject Headings

  • Xanthenes
  • Tomography, Optical
  • Reproducibility of Results
  • Phantoms, Imaging
  • Nuclear Medicine & Medical Imaging
  • Neoplasms, Experimental
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Light
 

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Thomas, A., Bowsher, J., Roper, J., Oliver, T., Dewhirst, M., & Oldham, M. (2010). A comprehensive method for optical-emission computed tomography. Phys Med Biol, 55(14), 3947–3957. https://doi.org/10.1088/0031-9155/55/14/001
Thomas, Andrew, James Bowsher, Justin Roper, Tim Oliver, Mark Dewhirst, and Mark Oldham. “A comprehensive method for optical-emission computed tomography.Phys Med Biol 55, no. 14 (July 21, 2010): 3947–57. https://doi.org/10.1088/0031-9155/55/14/001.
Thomas A, Bowsher J, Roper J, Oliver T, Dewhirst M, Oldham M. A comprehensive method for optical-emission computed tomography. Phys Med Biol. 2010 Jul 21;55(14):3947–57.
Thomas, Andrew, et al. “A comprehensive method for optical-emission computed tomography.Phys Med Biol, vol. 55, no. 14, July 2010, pp. 3947–57. Pubmed, doi:10.1088/0031-9155/55/14/001.
Thomas A, Bowsher J, Roper J, Oliver T, Dewhirst M, Oldham M. A comprehensive method for optical-emission computed tomography. Phys Med Biol. 2010 Jul 21;55(14):3947–3957.
Journal cover image

Published In

Phys Med Biol

DOI

EISSN

1361-6560

Publication Date

July 21, 2010

Volume

55

Issue

14

Start / End Page

3947 / 3957

Location

England

Related Subject Headings

  • Xanthenes
  • Tomography, Optical
  • Reproducibility of Results
  • Phantoms, Imaging
  • Nuclear Medicine & Medical Imaging
  • Neoplasms, Experimental
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Light