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Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.

Publication ,  Journal Article
Hansen, SJ; Rushton, J; Dekonenko, A; Chand, HS; Olson, GK; Hutt, JA; Pickup, D; Lyons, CR; Lipscomb, MF
Published in: Virology
April 10, 2011

Orthopoxviruses encode multiple proteins that modulate host immune responses. We determined whether cowpox virus (CPXV), a representative orthopoxvirus, modulated innate and acquired immune functions of human primary myeloid DCs and plasmacytoid DCs and monocyte-derived DCs (MDDCs). A CPXV infection of DCs at a multiplicity of infection of 10 was nonproductive, altered cellular morphology, and failed to reduce cell viability. A CPXV infection of DCs did not stimulate cytokine or chemokine secretion directly, but suppressed toll-like receptor (TLR) agonist-induced cytokine secretion and a DC-stimulated mixed leukocyte reaction (MLR). LPS-stimulated NF-κB nuclear translocation and host cytokine gene transcription were suppressed in CPXV-infected MDDCs. Early viral immunomodulatory genes were upregulated in MDDCs, consistent with early DC immunosuppression via synthesis of intracellular viral proteins. We conclude that a nonproductive CPXV infection suppressed DC immune function by synthesizing early intracellular viral proteins that suppressed DC signaling pathways.

Duke Scholars

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

April 10, 2011

Volume

412

Issue

2

Start / End Page

411 / 425

Location

United States

Related Subject Headings

  • Virology
  • NF-kappa B
  • Immune Evasion
  • Humans
  • Dendritic Cells
  • Cytokines
  • Cowpox virus
  • Cells, Cultured
  • Cell Survival
  • 32 Biomedical and clinical sciences
 

Citation

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Hansen, S. J., Rushton, J., Dekonenko, A., Chand, H. S., Olson, G. K., Hutt, J. A., … Lipscomb, M. F. (2011). Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection. Virology, 412(2), 411–425. https://doi.org/10.1016/j.virol.2011.01.024
Hansen, Spencer J., John Rushton, Alexander Dekonenko, Hitendra S. Chand, Gwyneth K. Olson, Julie A. Hutt, David Pickup, C Rick Lyons, and Mary F. Lipscomb. “Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.Virology 412, no. 2 (April 10, 2011): 411–25. https://doi.org/10.1016/j.virol.2011.01.024.
Hansen SJ, Rushton J, Dekonenko A, Chand HS, Olson GK, Hutt JA, et al. Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection. Virology. 2011 Apr 10;412(2):411–25.
Hansen, Spencer J., et al. “Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.Virology, vol. 412, no. 2, Apr. 2011, pp. 411–25. Pubmed, doi:10.1016/j.virol.2011.01.024.
Hansen SJ, Rushton J, Dekonenko A, Chand HS, Olson GK, Hutt JA, Pickup D, Lyons CR, Lipscomb MF. Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection. Virology. 2011 Apr 10;412(2):411–425.
Journal cover image

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

April 10, 2011

Volume

412

Issue

2

Start / End Page

411 / 425

Location

United States

Related Subject Headings

  • Virology
  • NF-kappa B
  • Immune Evasion
  • Humans
  • Dendritic Cells
  • Cytokines
  • Cowpox virus
  • Cells, Cultured
  • Cell Survival
  • 32 Biomedical and clinical sciences