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Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.

Publication ,  Journal Article
Jacobs, JP; Pettit, AR; Shinohara, ML; Jansson, M; Cantor, H; Gravallese, EM; Mathis, D; Benoist, C
Published in: Arthritis Rheum
August 2004

OBJECTIVE: Osteopontin (OPN) is a secreted glycoprotein involved in a range of physiologic processes, including inflammation, immunity mediated by Th1 cells, and bone remodeling. It is expressed in the joints of rheumatoid arthritis patients and has been the subject of conflicting reports concerning its role in arthritis induced by antibodies against type II collagen. This study assessed the role of OPN in the K/BxN serum-transfer model of autoantibody-induced arthritis. METHODS: Expression of OPN gene transcripts was assessed by microarray analysis of ankle RNA taken at 6 time points after transfer of K/BxN serum. OPN-sufficient or OPN-deficient littermates backcrossed for 10 generations onto the C57BL/6 genetic background were given K/BxN serum. Arthritis severity was measured by ankle thickening and a clinical index. Hind limb sections were stained with hematoxylin and eosin or toluidine blue and scored for inflammation, cartilage damage, and bone erosion. RESULTS: OPN messenger RNA transcripts progressively increased in ankle joints during the course of K/BxN serum-transferred arthritis. OPN-deficient mice receiving K/BxN serum developed arthritis with kinetics and clinical severity comparable with those of OPN-sufficient littermates. Histologic assessment of arthritic joints from OPN-deficient mice revealed synovial hyperplasia, pannus formation, mononuclear cell infiltration, bone erosion, cartilage damage at sites adjacent to and distal from pannus invasion, and tartrate-resistant acid phosphatase-positive multinucleated cells at sites of bone erosion. Histopathologic scoring demonstrated comparable levels of inflammation, cartilage damage, and bone erosion in OPN-sufficient and OPN-deficient mice. CONCLUSION: OPN does not have a required role in inflammation, bone erosion, and cartilage damage in the K/BxN serum-transfer model.

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Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

August 2004

Volume

50

Issue

8

Start / End Page

2685 / 2694

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sialoglycoproteins
  • Osteopontin
  • Oligonucleotide Array Sequence Analysis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Inflammation
  • Cartilage
  • Bone and Bones
 

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Jacobs, J. P., Pettit, A. R., Shinohara, M. L., Jansson, M., Cantor, H., Gravallese, E. M., … Benoist, C. (2004). Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum, 50(8), 2685–2694. https://doi.org/10.1002/art.20381
Jacobs, Jonathan P., Allison R. Pettit, Mari L. Shinohara, Marianne Jansson, Harvey Cantor, Ellen M. Gravallese, Diane Mathis, and Christophe Benoist. “Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.Arthritis Rheum 50, no. 8 (August 2004): 2685–94. https://doi.org/10.1002/art.20381.
Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, et al. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum. 2004 Aug;50(8):2685–94.
Jacobs, Jonathan P., et al. “Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.Arthritis Rheum, vol. 50, no. 8, Aug. 2004, pp. 2685–94. Pubmed, doi:10.1002/art.20381.
Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum. 2004 Aug;50(8):2685–2694.
Journal cover image

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

August 2004

Volume

50

Issue

8

Start / End Page

2685 / 2694

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sialoglycoproteins
  • Osteopontin
  • Oligonucleotide Array Sequence Analysis
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Inflammation
  • Cartilage
  • Bone and Bones