Skip to main content
Journal cover image

Detailed analysis of gene expression during development of T cell lineages in the thymus.

Publication ,  Journal Article
McCarty, N; Shinohara, ML; Lu, L; Cantor, H
Published in: Proc Natl Acad Sci U S A
June 22, 2004

The genetic mechanisms that promote lineage commitment and eliminate autoreactive cells in the thymus are not well understood. To better understand this process, we have identified and quantitated transcripts in the two major thymocyte lineages by using serial analysis of gene expression. Approximately 25 genes displayed almost complete segregation to one or the other T cell lineage. Commitment to the CD4 lineage was marked by up-regulation of genes associated with increased survival and chaperone function followed by expression of genes that regulate nucleosome remodeling and T cell receptor signaling. Differentiation within the CD8 lineage, on the other hand, was marked by up-regulation of genes that regulate lymphocyte homing, followed by quenching of genes that inhibit apoptosis. Definition of differential gene expression during development of the two major thymocyte lineages will allow insight into mechanisms of T cell development after positive and negative selection.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 22, 2004

Volume

101

Issue

25

Start / End Page

9339 / 9344

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Polymerase Chain Reaction
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Histocompatibility Antigens Class II
  • Histocompatibility Antigens Class I
  • Gene Library
  • Gene Expression Regulation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McCarty, N., Shinohara, M. L., Lu, L., & Cantor, H. (2004). Detailed analysis of gene expression during development of T cell lineages in the thymus. Proc Natl Acad Sci U S A, 101(25), 9339–9344. https://doi.org/10.1073/pnas.0402654101
McCarty, Nami, Mari L. Shinohara, Linrong Lu, and Harvey Cantor. “Detailed analysis of gene expression during development of T cell lineages in the thymus.Proc Natl Acad Sci U S A 101, no. 25 (June 22, 2004): 9339–44. https://doi.org/10.1073/pnas.0402654101.
McCarty N, Shinohara ML, Lu L, Cantor H. Detailed analysis of gene expression during development of T cell lineages in the thymus. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9339–44.
McCarty, Nami, et al. “Detailed analysis of gene expression during development of T cell lineages in the thymus.Proc Natl Acad Sci U S A, vol. 101, no. 25, June 2004, pp. 9339–44. Pubmed, doi:10.1073/pnas.0402654101.
McCarty N, Shinohara ML, Lu L, Cantor H. Detailed analysis of gene expression during development of T cell lineages in the thymus. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9339–9344.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 22, 2004

Volume

101

Issue

25

Start / End Page

9339 / 9344

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Polymerase Chain Reaction
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Histocompatibility Antigens Class II
  • Histocompatibility Antigens Class I
  • Gene Library
  • Gene Expression Regulation