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Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses.

Publication ,  Journal Article
Inoue, M; Moriwaki, Y; Arikawa, T; Chen, Y-H; Oh, YJ; Oliver, T; Shinohara, ML
Published in: J Immunol
January 1, 2011

We found that absence of osteopontin (OPN) in immunocompromised Rag2(-/-) mice, which lack T and B cells, made the mice extremely susceptible to an opportunistic fungus Pneumocystis, although immunocompetent OPN-deficient mice could clear Pneumocystis as well as wild-type mice. OPN has been studied as an extracellular protein, and the role of an intracellular isoform of OPN (iOPN) is still largely unknown. In this study, we elucidated the mechanism by which iOPN was involved in antifungal innate immunity. First, iOPN was essential for cluster formation of fungal receptors that detect Pneumocystis, including dectin-1, TLR2, and mannose receptor. Second, iOPN played a role as an adaptor molecule in TLR2 and dectin-1 signaling pathways and mediated ERK activation and cytokine production by zymosan, which simultaneously activates TLR2 and dectin-1 pathways. Third, iOPN enhanced phagocytosis and clearance of Pneumocystis. Our study suggests the critical involvement of iOPN in antifungal innate immunity.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

January 1, 2011

Volume

186

Issue

1

Start / End Page

19 / 23

Location

United States

Related Subject Headings

  • Toll-Like Receptor 2
  • Signal Transduction
  • Receptors, Pattern Recognition
  • Pneumocystis Infections
  • Pneumocystis
  • Osteopontin
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Inoue, M., Moriwaki, Y., Arikawa, T., Chen, Y.-H., Oh, Y. J., Oliver, T., & Shinohara, M. L. (2011). Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses. J Immunol, 186(1), 19–23. https://doi.org/10.4049/jimmunol.1002735
Inoue, Makoto, Yasuhiro Moriwaki, Tomohiro Arikawa, Yu-Hsun Chen, Young Joo Oh, Timothy Oliver, and Mari L. Shinohara. “Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses.J Immunol 186, no. 1 (January 1, 2011): 19–23. https://doi.org/10.4049/jimmunol.1002735.
Inoue M, Moriwaki Y, Arikawa T, Chen Y-H, Oh YJ, Oliver T, et al. Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses. J Immunol. 2011 Jan 1;186(1):19–23.
Inoue, Makoto, et al. “Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses.J Immunol, vol. 186, no. 1, Jan. 2011, pp. 19–23. Pubmed, doi:10.4049/jimmunol.1002735.
Inoue M, Moriwaki Y, Arikawa T, Chen Y-H, Oh YJ, Oliver T, Shinohara ML. Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses. J Immunol. 2011 Jan 1;186(1):19–23.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

January 1, 2011

Volume

186

Issue

1

Start / End Page

19 / 23

Location

United States

Related Subject Headings

  • Toll-Like Receptor 2
  • Signal Transduction
  • Receptors, Pattern Recognition
  • Pneumocystis Infections
  • Pneumocystis
  • Osteopontin
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice