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A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis.

Publication ,  Journal Article
St Clair, EW; Moak, SA; Wilkinson, WE; Sanders, L; Lang, T; Greenwald, RA
Published in: J Rheumatol
August 1998

OBJECTIVE: To compare 5 different assays measuring collagen degradation in rheumatoid arthritis (RA). METHODS: Daily serum samples and 3 consecutive 24 h urine samples were obtained from 25 patients with RA and 20 control subjects. Levels of pyridinoline (PYD), deoxypyridinoline (DPYD), n-telopeptide (NTx), CrossLaps (XL), and carboxy-terminal peptide of type I collagen (ICTP) were determined by ELISA or radioimmunoassay. PYD, DPYD, NTx, and XL were measured in urine and expressed as a ratio of the amount of crosslink to mmoles of creatinine (Cr). ICTP was determined in serum. The day-to-day variability of urinary collagen crosslink levels and serum ICTP was assessed over 3 day hospitalization. RESULTS: Four of the 5 markers were significantly elevated in the RA cohort compared to controls: PYD (nmol)/Cr (median 33.8 vs 19.3; p = 0.0001), NTx (nmol)/Cr (median 22.5 vs 13.8; p = 0.01), XL (microg)/Cr (median 191.4 vs 126.1; p = 0.01), and ICTP (microg/l) (median 5.8 vs 3.7; p = 0.001). In the RA group, higher levels of the markers were associated with concomitant prednisone therapy. The levels of the 4 urine markers and of ICTP in serum exhibited little day-to-day variability. CONCLUSION: Biochemical evidence of increased collagen degradation can be readily observed in RA using simple quantitative assays. These measures have minimal short term, day-to-day variability and hence may be useful to assess the effect of potentially disease modifying therapies.

Duke Scholars

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

August 1998

Volume

25

Issue

8

Start / End Page

1472 / 1479

Location

Canada

Related Subject Headings

  • Prednisone
  • Peptides
  • Peptide Fragments
  • Middle Aged
  • Male
  • Humans
  • Female
  • Cross-Sectional Studies
  • Collagen Type I
  • Collagen
 

Citation

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St Clair, E. W., Moak, S. A., Wilkinson, W. E., Sanders, L., Lang, T., & Greenwald, R. A. (1998). A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis. J Rheumatol, 25(8), 1472–1479.
St Clair, E. W., S. A. Moak, W. E. Wilkinson, L. Sanders, T. Lang, and R. A. Greenwald. “A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis.J Rheumatol 25, no. 8 (August 1998): 1472–79.
St Clair EW, Moak SA, Wilkinson WE, Sanders L, Lang T, Greenwald RA. A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis. J Rheumatol. 1998 Aug;25(8):1472–9.
St Clair, E. W., et al. “A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis.J Rheumatol, vol. 25, no. 8, Aug. 1998, pp. 1472–79.
St Clair EW, Moak SA, Wilkinson WE, Sanders L, Lang T, Greenwald RA. A cross sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis. J Rheumatol. 1998 Aug;25(8):1472–1479.

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

August 1998

Volume

25

Issue

8

Start / End Page

1472 / 1479

Location

Canada

Related Subject Headings

  • Prednisone
  • Peptides
  • Peptide Fragments
  • Middle Aged
  • Male
  • Humans
  • Female
  • Cross-Sectional Studies
  • Collagen Type I
  • Collagen