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In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA.

Publication ,  Journal Article
St Clair, EW; Burch, JA
Published in: Clin Immunol Immunopathol
April 1996

Autoantibodies to U1 RNA occur frequently in sera from patients with SLE and SLE-overlap syndromes. These autoantibodies have been previously shown to recognize major epitopes on stem-loops II and IV of U1 RNA. To further define these recognition sites, in vitro RNA selection was performed to identify the individual nucleotides which form the antibody binding site. Serum autoantibodies were used in this procedure to select synthetic variants from a library of RNA oligomers with a central region of 25 degenerate nucleotides in a linear context. A consensus sequence was derived from the autoantibody-selected RNA ligands that corresponded to a region of stem-loop II. It contained six continuous nucleotides (U63-C64-C65,-A66-X-U68) and one presumed discontinuous nucleotide (U79). In vitro selection of RNA ligands from simpler sublibraries consisting of oligomers with random loops and fixed U1 stem II sequences yielded no consensus sequence, suggesting that autoimmune recognition occurs independent of loop nucleotides. Competition assays confirmed the specificity of these binding reactions. The structural nature of this autoimmune U1 RNA epitope is compatible with a model of autoantibody production based on stimulation by native small nuclear ribonucleoprotein particles.

Duke Scholars

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1996

Volume

79

Issue

1

Start / End Page

60 / 70

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Ribonucleoprotein, U1 Small Nuclear
  • Rheumatic Diseases
  • RNA-Binding Proteins
  • RNA, Small Nuclear
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Immunology
  • Humans
  • Hela Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
St Clair, E. W., & Burch, J. A. (1996). In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA. Clin Immunol Immunopathol, 79(1), 60–70. https://doi.org/10.1006/clin.1996.0051
St Clair, E. W., and J. A. Burch. “In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA.Clin Immunol Immunopathol 79, no. 1 (April 1996): 60–70. https://doi.org/10.1006/clin.1996.0051.
St Clair EW, Burch JA. In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA. Clin Immunol Immunopathol. 1996 Apr;79(1):60–70.
St Clair, E. W., and J. A. Burch. “In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA.Clin Immunol Immunopathol, vol. 79, no. 1, Apr. 1996, pp. 60–70. Pubmed, doi:10.1006/clin.1996.0051.
St Clair EW, Burch JA. In vitro RNA selection of an autoimmune epitope on stem-loop II of U1 RNA. Clin Immunol Immunopathol. 1996 Apr;79(1):60–70.

Published In

Clin Immunol Immunopathol

DOI

ISSN

0090-1229

Publication Date

April 1996

Volume

79

Issue

1

Start / End Page

60 / 70

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Ribonucleoprotein, U1 Small Nuclear
  • Rheumatic Diseases
  • RNA-Binding Proteins
  • RNA, Small Nuclear
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Immunology
  • Humans
  • Hela Cells