Skip to main content

B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity.

Publication ,  Journal Article
Yanaba, K; Hamaguchi, Y; Venturi, GM; Steeber, DA; St Clair, EW; Tedder, TF
Published in: J Immunol
July 15, 2007

Rheumatoid arthritis is a systemic autoimmune disease. B cells are likely to play a critical role in arthritis pathogenesis, although it is unclear whether they are necessary for disease induction, autoantibody production, or disease progression. To assess the role of B cells in inflammatory arthritis, B cells were depleted using mouse anti-mouse CD20 mAbs in a mouse model of collagen-induced arthritis. CD20 mAbs effectively depleted mature B cells from adult DBA-1 mice. When B cells were depleted using CD20 mAbs before collagen immunization, there was a delay in disease onset and autoantibody production, with significantly diminished severity of arthritis both clinically and histologically. B cell depletion further delayed disease onset if initiated before, as well as after, collagen immunization. However, in both cases, the eventual reappearance of peripheral B cells triggered autoantibody production and the subsequent development of arthritis in collagen-sensitized mice. By contrast, B cell depletion after collagen immunizations did not have a significant effect on arthritis progression or severity. Thus, disease symptoms were only induced when peripheral B cells and their autoantibody products were present in collagen-immunized mice, documenting a critical role for B cells during the elicitation phase of collagen-induced arthritis. These studies suggest that B cell depletion strategies will be most effective when initiated early in the development of inflammatory arthritis, with sustained B cell depletion required to inhibit the production of isotype-switched pathogenic Abs and the evolution of joint inflammation and destruction.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 15, 2007

Volume

179

Issue

2

Start / End Page

1369 / 1380

Location

United States

Related Subject Headings

  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Depletion
  • Immunology
  • Immunity, Cellular
  • Enzyme-Linked Immunosorbent Assay
  • Disease Progression
  • B-Lymphocytes
  • Autoantibodies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yanaba, K., Hamaguchi, Y., Venturi, G. M., Steeber, D. A., St Clair, E. W., & Tedder, T. F. (2007). B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity. J Immunol, 179(2), 1369–1380. https://doi.org/10.4049/jimmunol.179.2.1369
Yanaba, Koichi, Yasuhito Hamaguchi, Guglielmo M. Venturi, Douglas A. Steeber, E William St Clair, and Thomas F. Tedder. “B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity.J Immunol 179, no. 2 (July 15, 2007): 1369–80. https://doi.org/10.4049/jimmunol.179.2.1369.
Yanaba K, Hamaguchi Y, Venturi GM, Steeber DA, St Clair EW, Tedder TF. B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity. J Immunol. 2007 Jul 15;179(2):1369–80.
Yanaba, Koichi, et al. “B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity.J Immunol, vol. 179, no. 2, July 2007, pp. 1369–80. Pubmed, doi:10.4049/jimmunol.179.2.1369.
Yanaba K, Hamaguchi Y, Venturi GM, Steeber DA, St Clair EW, Tedder TF. B cell depletion delays collagen-induced arthritis in mice: arthritis induction requires synergy between humoral and cell-mediated immunity. J Immunol. 2007 Jul 15;179(2):1369–1380.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 15, 2007

Volume

179

Issue

2

Start / End Page

1369 / 1380

Location

United States

Related Subject Headings

  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Depletion
  • Immunology
  • Immunity, Cellular
  • Enzyme-Linked Immunosorbent Assay
  • Disease Progression
  • B-Lymphocytes
  • Autoantibodies