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Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development.

Publication ,  Journal Article
Peralta, XG; Toyama, Y; Hutson, MS; Montague, R; Venakides, S; Kiehart, DP; Edwards, GS
Published in: Biophysical journal
April 2007

Tissue dynamics during dorsal closure, a stage of Drosophila development, provide a model system for cell sheet morphogenesis and wound healing. Dorsal closure is characterized by complex cell sheet movements, driven by multiple tissue specific forces, which are coordinated in space, synchronized in time, and resilient to UV-laser perturbations. The mechanisms responsible for these attributes are not fully understood. We measured spatial, kinematic, and dynamic antero-posterior asymmetries to biophysically characterize both resiliency to laser perturbations and failure of closure in mutant embryos and compared them to natural asymmetries in unperturbed, wild-type closure. We quantified and mathematically modeled two processes that are upregulated to provide resiliency--contractility of the amnioserosa and formation of a seam between advancing epidermal sheets, i.e., zipping. Both processes are spatially removed from the laser-targeted site, indicating they are not a local response to laser-induced wounding and suggesting mechanosensitive and/or chemosensitive mechanisms for upregulation. In mutant embryos, tissue junctions initially fail at the anterior end indicating inhomogeneous mechanical stresses attributable to head involution, another developmental process that occurs concomitant with the end stages of closure. Asymmetries in these mutants are reversed compared to wild-type, and inhomogeneous stresses may cause asymmetries in wild-type closure.

Duke Scholars

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2007

Volume

92

Issue

7

Start / End Page

2583 / 2596

Related Subject Headings

  • Up-Regulation
  • Stress, Mechanical
  • Morphogenesis
  • Models, Biological
  • Mechanotransduction, Cellular
  • Drosophila
  • Computer Simulation
  • Biophysics
  • Animals
  • 51 Physical sciences
 

Citation

APA
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ICMJE
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Peralta, X. G., Toyama, Y., Hutson, M. S., Montague, R., Venakides, S., Kiehart, D. P., & Edwards, G. S. (2007). Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development. Biophysical Journal, 92(7), 2583–2596. https://doi.org/10.1529/biophysj.106.094110
Peralta, X. G., Y. Toyama, M. S. Hutson, R. Montague, S. Venakides, D. P. Kiehart, and G. S. Edwards. “Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development.Biophysical Journal 92, no. 7 (April 2007): 2583–96. https://doi.org/10.1529/biophysj.106.094110.
Peralta XG, Toyama Y, Hutson MS, Montague R, Venakides S, Kiehart DP, et al. Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development. Biophysical journal. 2007 Apr;92(7):2583–96.
Peralta, X. G., et al. “Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development.Biophysical Journal, vol. 92, no. 7, Apr. 2007, pp. 2583–96. Epmc, doi:10.1529/biophysj.106.094110.
Peralta XG, Toyama Y, Hutson MS, Montague R, Venakides S, Kiehart DP, Edwards GS. Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development. Biophysical journal. 2007 Apr;92(7):2583–2596.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2007

Volume

92

Issue

7

Start / End Page

2583 / 2596

Related Subject Headings

  • Up-Regulation
  • Stress, Mechanical
  • Morphogenesis
  • Models, Biological
  • Mechanotransduction, Cellular
  • Drosophila
  • Computer Simulation
  • Biophysics
  • Animals
  • 51 Physical sciences