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Forces for morphogenesis investigated with laser microsurgery and quantitative modeling.

Publication ,  Journal Article
Hutson, MS; Tokutake, Y; Chang, M-S; Bloor, JW; Venakides, S; Kiehart, DP; Edwards, GS
Published in: Science (New York, N.Y.)
April 2003

We investigated the forces that connect the genetic program of development to morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for development and wound healing. We found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages. We applied quantitative modeling to show that these forces, generated in distinct cells, are coordinated in space and synchronized in time. Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.

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Published In

Science (New York, N.Y.)

DOI

EISSN

1095-9203

ISSN

0036-8075

Publication Date

April 2003

Volume

300

Issue

5616

Start / End Page

145 / 149

Related Subject Headings

  • Pseudopodia
  • Mutation
  • Morphogenesis
  • Models, Biological
  • Microsurgery
  • Microscopy, Confocal
  • Mathematics
  • Lasers
  • Integrins
  • Integrin alpha Chains
 

Citation

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Hutson, M. S., Tokutake, Y., Chang, M.-S., Bloor, J. W., Venakides, S., Kiehart, D. P., & Edwards, G. S. (2003). Forces for morphogenesis investigated with laser microsurgery and quantitative modeling. Science (New York, N.Y.), 300(5616), 145–149. https://doi.org/10.1126/science.1079552
Hutson, M Shane, Yoichiro Tokutake, Ming-Shien Chang, James W. Bloor, Stephanos Venakides, Daniel P. Kiehart, and Glenn S. Edwards. “Forces for morphogenesis investigated with laser microsurgery and quantitative modeling.Science (New York, N.Y.) 300, no. 5616 (April 2003): 145–49. https://doi.org/10.1126/science.1079552.
Hutson MS, Tokutake Y, Chang M-S, Bloor JW, Venakides S, Kiehart DP, et al. Forces for morphogenesis investigated with laser microsurgery and quantitative modeling. Science (New York, NY). 2003 Apr;300(5616):145–9.
Hutson, M. Shane, et al. “Forces for morphogenesis investigated with laser microsurgery and quantitative modeling.Science (New York, N.Y.), vol. 300, no. 5616, Apr. 2003, pp. 145–49. Epmc, doi:10.1126/science.1079552.
Hutson MS, Tokutake Y, Chang M-S, Bloor JW, Venakides S, Kiehart DP, Edwards GS. Forces for morphogenesis investigated with laser microsurgery and quantitative modeling. Science (New York, NY). 2003 Apr;300(5616):145–149.
Journal cover image

Published In

Science (New York, N.Y.)

DOI

EISSN

1095-9203

ISSN

0036-8075

Publication Date

April 2003

Volume

300

Issue

5616

Start / End Page

145 / 149

Related Subject Headings

  • Pseudopodia
  • Mutation
  • Morphogenesis
  • Models, Biological
  • Microsurgery
  • Microscopy, Confocal
  • Mathematics
  • Lasers
  • Integrins
  • Integrin alpha Chains