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Drosophilia spectrin. I. Characterization of the purified protein.

Publication ,  Journal Article
Dubreuil, R; Byers, TJ; Branton, D; Goldstein, LS; Kiehart, DP
Published in: The Journal of cell biology
November 1987

We purified a protein from Drosophila S3 tissue culture cells that has many of the diagnostic features of spectrin from vertebrate organisms: (a) The protein consists of two equimolar subunits (Mr = 234 and 226 kD) that can be reversibly cross-linked into a complex composed of equal amounts of the two subunits. (b) Electron microscopy of the native molecule reveals two intertwined, elongated strands with a contour length of 180 nm. (c) Antibodies directed against vertebrate spectrin react with the Drosophila protein and, similarly, antibodies to the Drosophila protein react with vertebrate spectrins. One monoclonal antibody has been found to react with both of the Drosophila subunits and with both subunits of vertebrate brain spectrin. (d) The Drosophila protein exhibits both actin-binding and calcium-dependent calmodulin-binding activities. Based on the above criteria, this protein appears to be a bona fide member of the spectrin family of proteins.

Duke Scholars

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

November 1987

Volume

105

Issue

5

Start / End Page

2095 / 2102

Related Subject Headings

  • Spectrin
  • Molecular Weight
  • Microscopy, Electron
  • Macromolecular Substances
  • Drosophila
  • Developmental Biology
  • Cell Line
  • Calmodulin
  • Antigen-Antibody Complex
  • Antibodies, Monoclonal
 

Citation

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Dubreuil, R., Byers, T. J., Branton, D., Goldstein, L. S., & Kiehart, D. P. (1987). Drosophilia spectrin. I. Characterization of the purified protein. The Journal of Cell Biology, 105(5), 2095–2102. https://doi.org/10.1083/jcb.105.5.2095
Dubreuil, R., T. J. Byers, D. Branton, L. S. Goldstein, and D. P. Kiehart. “Drosophilia spectrin. I. Characterization of the purified protein.The Journal of Cell Biology 105, no. 5 (November 1987): 2095–2102. https://doi.org/10.1083/jcb.105.5.2095.
Dubreuil R, Byers TJ, Branton D, Goldstein LS, Kiehart DP. Drosophilia spectrin. I. Characterization of the purified protein. The Journal of cell biology. 1987 Nov;105(5):2095–102.
Dubreuil, R., et al. “Drosophilia spectrin. I. Characterization of the purified protein.The Journal of Cell Biology, vol. 105, no. 5, Nov. 1987, pp. 2095–102. Epmc, doi:10.1083/jcb.105.5.2095.
Dubreuil R, Byers TJ, Branton D, Goldstein LS, Kiehart DP. Drosophilia spectrin. I. Characterization of the purified protein. The Journal of cell biology. 1987 Nov;105(5):2095–2102.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

November 1987

Volume

105

Issue

5

Start / End Page

2095 / 2102

Related Subject Headings

  • Spectrin
  • Molecular Weight
  • Microscopy, Electron
  • Macromolecular Substances
  • Drosophila
  • Developmental Biology
  • Cell Line
  • Calmodulin
  • Antigen-Antibody Complex
  • Antibodies, Monoclonal