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Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure.

Publication ,  Journal Article
Layton, AT; Toyama, Y; Yang, G-Q; Edwards, GS; Kiehart, DP; Venakides, S
Published in: HFSP journal
December 2009

Dorsal closure, a stage of Drosophila development, is a model system for cell sheet morphogenesis and wound healing. During closure, two flanks of epidermal tissue progressively advance to reduce the area of the eye-shaped opening in the dorsal surface, which contains amnioserosa tissue. To simulate the time evolution of the overall shape of the dorsal opening, we developed a mathematical model, in which contractility and elasticity are manifest in model force-producing elements that satisfy force-velocity relationships similar to muscle. The action of the elements is consistent with the force-producing behavior of actin and myosin in cells. The parameters that characterize the simulated embryos were optimized by reference to experimental observations on wild-type embryos and, to a lesser extent, on embryos whose amnioserosa was removed by laser surgery and on myospheroid mutant embryos. Simulations failed to reproduce the amnioserosa-removal protocol in either the elastic or the contractile limit, indicating that both elastic and contractile dynamics are essential components of the biological force-producing elements. We found it was necessary to actively upregulate forces to recapitulate both the double and single-canthus nick protocols, which did not participate in the optimization of parameters, suggesting the existence of additional key feedback mechanisms.

Duke Scholars

Published In

HFSP journal

DOI

EISSN

1955-205X

ISSN

1955-2068

Publication Date

December 2009

Volume

3

Issue

6

Start / End Page

441 / 460

Related Subject Headings

  • Developmental Biology
 

Citation

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Layton, A. T., Toyama, Y., Yang, G.-Q., Edwards, G. S., Kiehart, D. P., & Venakides, S. (2009). Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure. HFSP Journal, 3(6), 441–460. https://doi.org/10.2976/1.3266062
Layton, Anita T., Yusuke Toyama, Guo-Qiang Yang, Glenn S. Edwards, Daniel P. Kiehart, and Stephanos Venakides. “Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure.HFSP Journal 3, no. 6 (December 2009): 441–60. https://doi.org/10.2976/1.3266062.
Layton AT, Toyama Y, Yang G-Q, Edwards GS, Kiehart DP, Venakides S. Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure. HFSP journal. 2009 Dec;3(6):441–60.
Layton, Anita T., et al. “Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure.HFSP Journal, vol. 3, no. 6, Dec. 2009, pp. 441–60. Epmc, doi:10.2976/1.3266062.
Layton AT, Toyama Y, Yang G-Q, Edwards GS, Kiehart DP, Venakides S. Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure. HFSP journal. 2009 Dec;3(6):441–460.

Published In

HFSP journal

DOI

EISSN

1955-205X

ISSN

1955-2068

Publication Date

December 2009

Volume

3

Issue

6

Start / End Page

441 / 460

Related Subject Headings

  • Developmental Biology