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Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA.

Publication ,  Journal Article
Pisetsky, DS; Grudier, JP
Published in: J Immunol
December 1, 1989

To characterize further polyspecific interactions of antibodies to DNA, the binding of sera from autoimmune MRL-lpr/lpr mice to Escherichia coli beta-galactosidase (beta-gal) was analyzed. This protein was selected for study because of preliminary observations that sera from autoimmune mice bound unexpectedly to cloned fusion protein constructions containing beta-gal. Using ELISA assays, sera from MRL-lpr/lpr mice demonstrated high levels of antibodies to both DNA and beta-gal, in titers significantly greater than those of BALB/c controls. Affinity chromatography using beta-gal-Sepharose demonstrated that antibodies enriched for anti-beta-gal activity bound both DNA as well as beta-gal, indicating the presence of a population of cross-reactive anti-DNA antibodies. Furthermore, anti-DNA mAb of MRL-lpr/lpr strain origin also bound beta-gal by ELISA, although these levels were lower than those to DNA. Together, these results extend the range of polyspecific binding of murine anti-DNA antibodies to bacterial proteins. They further suggest caution in the interpretation of immunoassays using fusion protein constructions containing beta-gal, especially with sera from autoimmune mice.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1, 1989

Volume

143

Issue

11

Start / End Page

3609 / 3613

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Recombinant Fusion Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • Galactosidases
  • Escherichia coli
 

Citation

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Pisetsky, D. S., & Grudier, J. P. (1989). Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA. J Immunol, 143(11), 3609–3613.
Pisetsky, D. S., and J. P. Grudier. “Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA.J Immunol 143, no. 11 (December 1, 1989): 3609–13.
Pisetsky DS, Grudier JP. Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA. J Immunol. 1989 Dec 1;143(11):3609–13.
Pisetsky, D. S., and J. P. Grudier. “Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA.J Immunol, vol. 143, no. 11, Dec. 1989, pp. 3609–13.
Pisetsky DS, Grudier JP. Polyspecific binding of Escherichia coli beta-galactosidase by murine antibodies to DNA. J Immunol. 1989 Dec 1;143(11):3609–3613.

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1, 1989

Volume

143

Issue

11

Start / End Page

3609 / 3613

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Recombinant Fusion Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lupus Erythematosus, Systemic
  • Immunology
  • Humans
  • Galactosidases
  • Escherichia coli