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Idiotypic analysis of a monoclonal anti-Sm antibody. II. Strain distribution of a common idiotypic determinant and its relationship to anti-Sm expression.

Publication ,  Journal Article
Pisetsky, DS; Semper, KF; Eisenberg, RA
Published in: J Immunol
October 1984

The idiotypes borne by Y2, a monoclonal anti-Sm antibody of MRL-lpr/lpr mouse strain origin, were investigated to elucidate genetic mechanisms in this autoantibody response. An anti-Y2 anti-idiotypic antiserum was raised in a rabbit and was rendered specific for idiotype by extensive absorption with globulins of the B6 and BALB/c strains as well as the BALB/c myeloma UPC 10. By using a sensitive assay for idiotype by inhibition ELISA, the Y2 determinant was found to be commonly expressed in sera of MRL-lpr/lpr and MRL-+/+ mice. Moreover, sera of several normal strain mice also bore the idiotype and, in mice bearing the lpr gene, idiotype levels were increased 1.5 to fivefold, even in the absence of a serum anti-Sm response. The relationship of this idiotype to anti-Sm expression was further assessed by determining the idiotype content of affinity-purified anti-Sm antibodies from MRL-lpr/lpr mice. Anti-Sm from serum pools or individual animals showed no significant enrichment of the Y2 idiotype in comparison to unselected MRL-lpr/lpr IgG. These results suggest that the Y2 idiotype defines only a minor component of the anti-Sm autoantibody response, and that most antibodies with this determinant express other antigenic specificities.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1984

Volume

133

Issue

4

Start / End Page

2085 / 2089

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Species Specificity
  • Ribonucleoproteins, Small Nuclear
  • Rabbits
  • Mice, Mutant Strains
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
 

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1984

Volume

133

Issue

4

Start / End Page

2085 / 2089

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Species Specificity
  • Ribonucleoproteins, Small Nuclear
  • Rabbits
  • Mice, Mutant Strains
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C