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The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage.

Publication ,  Journal Article
Bloom, DD; St Clair, EW; Pisetsky, DS; Clarke, SH
Published in: Eur J Immunol
June 1994

Autoantibodies to ribonucleoproteins (RNP) occur prominently in human systemic lupus erythematosus and murine lupus models. In previous studies we demonstrated a relationship in MRL/Mp-lpr/lpr (MRL/lpr) mice between antibodies to Sm, an RNP autoantigen, and antibodies to DNA. Thus, many anti-Sm monoclonals bound DNA and expressed the same V region genes as anti-DNA. In addition, many had multiple VHCDR3 Arg residues suggestive of selection by DNA, and some had somatic mutations suggesting selection for mutant B cells by DNA. To determine whether autoantibodies to other RNP antigens are also associated with the anti-DNA response, we have analyzed the response to the La RNP. Six anti-La B cell hybridomas were generated from a single MRL/lpr mouse. Southern blot analysis of Ig V gene rearrangements and V gene sequences indicated two clonally related pairs, suggesting an oligoclonal response. Antibodies from all six hybridomas bound single-stranded DNA, while antibodies from five hybridomas bound double-stranded DNA. Two hybridomas expressed a VH7183 gene which is used by members of two previously reported anti-DNA clones and two anti-Sm/DNA clones of MRL/lpr origin. These data demonstrate an association between the anti-La and anti-DNA responses in MRL/lpr mice, suggesting that cross-reactive anti-RNP and anti-DNA responses are a general feature of autoimmunity in this lupus model.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

June 1994

Volume

24

Issue

6

Start / End Page

1332 / 1338

Location

Germany

Related Subject Headings

  • snRNP Core Proteins
  • SS-B Antigen
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • Precipitin Tests
  • Molecular Sequence Data
  • Mice, Inbred Strains
  • Mice
  • Immunology
  • Immunoglobulin Variable Region
 

Citation

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Bloom, D. D., St Clair, E. W., Pisetsky, D. S., & Clarke, S. H. (1994). The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. Eur J Immunol, 24(6), 1332–1338. https://doi.org/10.1002/eji.1830240614
Bloom, D. D., E. W. St Clair, D. S. Pisetsky, and S. H. Clarke. “The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage.Eur J Immunol 24, no. 6 (June 1994): 1332–38. https://doi.org/10.1002/eji.1830240614.
Bloom DD, St Clair EW, Pisetsky DS, Clarke SH. The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. Eur J Immunol. 1994 Jun;24(6):1332–8.
Bloom, D. D., et al. “The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage.Eur J Immunol, vol. 24, no. 6, June 1994, pp. 1332–38. Pubmed, doi:10.1002/eji.1830240614.
Bloom DD, St Clair EW, Pisetsky DS, Clarke SH. The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. Eur J Immunol. 1994 Jun;24(6):1332–1338.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

June 1994

Volume

24

Issue

6

Start / End Page

1332 / 1338

Location

Germany

Related Subject Headings

  • snRNP Core Proteins
  • SS-B Antigen
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • Precipitin Tests
  • Molecular Sequence Data
  • Mice, Inbred Strains
  • Mice
  • Immunology
  • Immunoglobulin Variable Region