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Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.

Publication ,  Journal Article
St Clair, EW; Pisetsky, DS; Reich, CF; Chambers, JC; Keene, JD
Published in: Arthritis Rheum
April 1988

A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.

Duke Scholars

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

April 1988

Volume

31

Issue

4

Start / End Page

506 / 514

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • SS-B Antigen
  • Ribonucleoproteins
  • Humans
  • Escherichia coli
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel
  • Bacterial Proteins
  • Autoantigens
  • Arthritis & Rheumatology
 

Citation

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ICMJE
MLA
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St Clair, E. W., Pisetsky, D. S., Reich, C. F., Chambers, J. C., & Keene, J. D. (1988). Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. Arthritis Rheum, 31(4), 506–514. https://doi.org/10.1002/art.1780310407
St Clair, E. W., D. S. Pisetsky, C. F. Reich, J. C. Chambers, and J. D. Keene. “Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.Arthritis Rheum 31, no. 4 (April 1988): 506–14. https://doi.org/10.1002/art.1780310407.
St Clair EW, Pisetsky DS, Reich CF, Chambers JC, Keene JD. Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. Arthritis Rheum. 1988 Apr;31(4):506–14.
St Clair, E. W., et al. “Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen.Arthritis Rheum, vol. 31, no. 4, Apr. 1988, pp. 506–14. Pubmed, doi:10.1002/art.1780310407.
St Clair EW, Pisetsky DS, Reich CF, Chambers JC, Keene JD. Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. Arthritis Rheum. 1988 Apr;31(4):506–514.
Journal cover image

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

April 1988

Volume

31

Issue

4

Start / End Page

506 / 514

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • SS-B Antigen
  • Ribonucleoproteins
  • Humans
  • Escherichia coli
  • Enzyme-Linked Immunosorbent Assay
  • Electrophoresis, Polyacrylamide Gel
  • Bacterial Proteins
  • Autoantigens
  • Arthritis & Rheumatology