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Temporal correlation of antibody responses to different epitopes of the human La autoantigen.

Publication ,  Journal Article
St Clair, EW; Burch, JA; Ward, MM; Keene, JD; Pisetsky, DS
Published in: J Clin Invest
February 1990

To investigate the temporal relationship of antibody responses to different La epitopes, sequential sera from nine patients with systemic lupus erythematosus and Sjogren's syndrome were tested by enzyme-linked immunosorbent assay for antibody binding to a series of recombinant fusion proteins containing different regions of the La molecule. The results of this analysis indicate that antibody responses to four different La fragments vary in parallel over time. This finding is supported by a statistical analysis indicating that the changes in antibody levels between the six pairs of responses were highly correlated (P less than 0.001). Furthermore, we show by immunoaffinity purification that antibodies to the three nonoverlapping La protein fragments do not cross-react with other fragments and, hence, represent independent populations. These results suggest that anti-La antibodies are coordinately produced to different epitopes on the La molecule, possibly reflecting an antigen-driven mechanism.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

February 1990

Volume

85

Issue

2

Start / End Page

515 / 521

Location

United States

Related Subject Headings

  • SS-B Antigen
  • Ribonucleoproteins
  • Peptide Fragments
  • Immunology
  • Immunoglobulin G
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Cross Reactions
 

Citation

APA
Chicago
ICMJE
MLA
NLM
St Clair, E. W., Burch, J. A., Ward, M. M., Keene, J. D., & Pisetsky, D. S. (1990). Temporal correlation of antibody responses to different epitopes of the human La autoantigen. J Clin Invest, 85(2), 515–521. https://doi.org/10.1172/JCI114467
St Clair, E. W., J. A. Burch, M. M. Ward, J. D. Keene, and D. S. Pisetsky. “Temporal correlation of antibody responses to different epitopes of the human La autoantigen.J Clin Invest 85, no. 2 (February 1990): 515–21. https://doi.org/10.1172/JCI114467.
St Clair EW, Burch JA, Ward MM, Keene JD, Pisetsky DS. Temporal correlation of antibody responses to different epitopes of the human La autoantigen. J Clin Invest. 1990 Feb;85(2):515–21.
St Clair, E. W., et al. “Temporal correlation of antibody responses to different epitopes of the human La autoantigen.J Clin Invest, vol. 85, no. 2, Feb. 1990, pp. 515–21. Pubmed, doi:10.1172/JCI114467.
St Clair EW, Burch JA, Ward MM, Keene JD, Pisetsky DS. Temporal correlation of antibody responses to different epitopes of the human La autoantigen. J Clin Invest. 1990 Feb;85(2):515–521.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

February 1990

Volume

85

Issue

2

Start / End Page

515 / 521

Location

United States

Related Subject Headings

  • SS-B Antigen
  • Ribonucleoproteins
  • Peptide Fragments
  • Immunology
  • Immunoglobulin G
  • Humans
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • DNA, Single-Stranded
  • Cross Reactions