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Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium.

Publication ,  Journal Article
Katz, ML; Drea, CM; Eldred, GE; Hess, HH; Robison, WG
Published in: Experimental eye research
October 1986

Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscin-like fluorescence of the outer-segment fragments in the RDY rats.

Duke Scholars

Published In

Experimental eye research

DOI

EISSN

1096-0007

ISSN

0014-4835

Publication Date

October 1986

Volume

43

Issue

4

Start / End Page

561 / 573

Related Subject Headings

  • Retinal Degeneration
  • Retina
  • Rats, Inbred Strains
  • Rats
  • Pigments, Biological
  • Pigment Epithelium of Eye
  • Photoreceptor Cells
  • Ophthalmology & Optometry
  • Microscopy, Electron
  • Male
 

Citation

APA
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MLA
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Katz, M. L., Drea, C. M., Eldred, G. E., Hess, H. H., & Robison, W. G. (1986). Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium. Experimental Eye Research, 43(4), 561–573. https://doi.org/10.1016/s0014-4835(86)80023-9
Katz, M. L., C. M. Drea, G. E. Eldred, H. H. Hess, and W. G. Robison. “Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium.Experimental Eye Research 43, no. 4 (October 1986): 561–73. https://doi.org/10.1016/s0014-4835(86)80023-9.
Katz ML, Drea CM, Eldred GE, Hess HH, Robison WG. Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium. Experimental eye research. 1986 Oct;43(4):561–73.
Katz, M. L., et al. “Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium.Experimental Eye Research, vol. 43, no. 4, Oct. 1986, pp. 561–73. Epmc, doi:10.1016/s0014-4835(86)80023-9.
Katz ML, Drea CM, Eldred GE, Hess HH, Robison WG. Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium. Experimental eye research. 1986 Oct;43(4):561–573.
Journal cover image

Published In

Experimental eye research

DOI

EISSN

1096-0007

ISSN

0014-4835

Publication Date

October 1986

Volume

43

Issue

4

Start / End Page

561 / 573

Related Subject Headings

  • Retinal Degeneration
  • Retina
  • Rats, Inbred Strains
  • Rats
  • Pigments, Biological
  • Pigment Epithelium of Eye
  • Photoreceptor Cells
  • Ophthalmology & Optometry
  • Microscopy, Electron
  • Male