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Murine CD7 shares antigenic cross-reactivity with HSP-60.

Publication ,  Journal Article
Howard, BA; Sempowski, GD; Scearce, RM; Liao, H-X; Lee, DM; Lam, GK; Chen, H; Fadden, P; Haystead, T; Haynes, BF
Published in: Hybridoma (Larchmt)
April 2008
Published version (DOI) Link to item

Human (h) CD7 is a 40 kDa single chain Ig superfamily molecule that is expressed on thymocytes, a major subunit of peripheral T cells, and most natural killer cells. Ligands for hCD7 include the epithelial cell-produced molecule, K-12, and galectin. Mice deficient in CD7 have been shown to be resistant to LPS-induced endotoxic shock syndromes. However, monoclonal antibodies (MAb) to mouse (m) CD7 have yet to be produced, nor is the distribution of mCD7 protein in mice known. We have raised a panel of three rat MAbs to mCD7 by immunizing rats with recombinant mCD7 protein. However, using Western blot and immunoprecipitation of tissue extracts from mouse thymus, spleen, liver, brain, lymph node and skin, these anti-mouse CD7 MAbs bound only to murine heat shock protein 60 (HSP-60) present both in wild-type (CD7+/+) and CD7-deficient (CD7-/-) mice. Epitope mapping of the sites on HSP-60 and recombinant mCD7 recognized by mCD7 MAbs demonstrated non-homologous amino acid sequence epitopes recognized by anti-CD7 MAbs on both proteins. These data demonstrated molecular mimicry of mCD7 with HSP-60, and leave open the question of surface expression of mCD7.

Duke Scholars

Brandon Augustus Howard
Author Brandon Augustus Howard Radiology, Nuclear Medicine
Timothy Arthur James Haystead
Author Timothy Arthur James Haystead Pharmacology & Cancer Biology
Barton Ford Haynes
Author Barton Ford Haynes Medicine, Duke Human Vaccine Institute

Published In

Hybridoma (Larchmt)

DOI

10.1089/hyb.2007.0553

ISSN

1554-0014

Publication Date

April 2008

Volume

27

Issue

2

Start / End Page

81 / 89

Location

United States

Related Subject Headings

  • Spleen
  • Rats
  • Rabbits
  • Organ Specificity
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Intestine, Small
  • Immunology
 

Citation

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MLA
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Howard, B. A., Sempowski, G. D., Scearce, R. M., Liao, H.-X., Lee, D. M., Lam, G. K., … Haynes, B. F. (2008). Murine CD7 shares antigenic cross-reactivity with HSP-60. Hybridoma (Larchmt), 27(2), 81–89. https://doi.org/10.1089/hyb.2007.0553
Howard, Brandon A., Gregory D. Sempowski, Richard M. Scearce, Hua-Xin Liao, David M. Lee, Gordon K. Lam, Haiyen Chen, Patrick Fadden, Timothy Haystead, and Barton F. Haynes. “Murine CD7 shares antigenic cross-reactivity with HSP-60.” Hybridoma (Larchmt) 27, no. 2 (April 2008): 81–89. https://doi.org/10.1089/hyb.2007.0553.
Howard BA, Sempowski GD, Scearce RM, Liao H-X, Lee DM, Lam GK, et al. Murine CD7 shares antigenic cross-reactivity with HSP-60. Hybridoma (Larchmt). 2008 Apr;27(2):81–9.
Howard, Brandon A., et al. “Murine CD7 shares antigenic cross-reactivity with HSP-60.” Hybridoma (Larchmt), vol. 27, no. 2, Apr. 2008, pp. 81–89. Pubmed, doi:10.1089/hyb.2007.0553.
Howard BA, Sempowski GD, Scearce RM, Liao H-X, Lee DM, Lam GK, Chen H, Fadden P, Haystead T, Haynes BF. Murine CD7 shares antigenic cross-reactivity with HSP-60. Hybridoma (Larchmt). 2008 Apr;27(2):81–89.

Published In

Hybridoma (Larchmt)

DOI

10.1089/hyb.2007.0553

ISSN

1554-0014

Publication Date

April 2008

Volume

27

Issue

2

Start / End Page

81 / 89

Location

United States

Related Subject Headings

  • Spleen
  • Rats
  • Rabbits
  • Organ Specificity
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Intestine, Small
  • Immunology