Skip to main content
Journal cover image

Ancestral and consensus envelope immunogens for HIV-1 subtype C.

Publication ,  Journal Article
Kothe, DL; Li, Y; Decker, JM; Bibollet-Ruche, F; Zammit, KP; Salazar, MG; Chen, Y; Weng, Z; Weaver, EA; Gao, F; Haynes, BF; Shaw, GM ...
Published in: Virology
September 1, 2006

Immunogens based on "centralized" (ancestral or consensus) HIV-1 sequences minimize the genetic distance between vaccine strains and contemporary viruses and should thus elicit immune responses that recognize a broader spectrum of viral variants. However, the biologic, antigenic and immunogenic properties of such inferred gene products have to be validated experimentally. Here, we report the construction and characterization of the first full-length ancestral (AncC) and consensus (ConC) env genes of HIV-1 (group M) subtype C. The codon-usage-optimized genes expressed high levels of envelope glycoproteins that were incorporated into HIV-1 virions, mediated infection via the CCR5 co-receptor and retained neutralizing epitopes as recognized by plasma from patients with chronic HIV-1 subtype C infection. Guinea pigs immunized with AncC and ConC env DNA developed high titer binding, but no appreciable homologous or heterologous neutralizing antibodies. When tested by immunoblot analysis, sera from AncC and ConC env immunized guinea pigs recognized a greater number of primary subtype C envelope glycoproteins than sera from guinea pigs immunized with a contemporary subtype C env control. Mice immunized with AncC and ConC env DNA developed gamma interferon T cell responses that recognized overlapping peptides from the cognate ConC and a heterologous subtype C Env control. Thus, both AncC and ConC env genes expressed functional envelope glycoproteins that were immunogenic in laboratory animals and elicited humoral and cellular immune responses of comparable breadth and magnitude. These results establish the utility of centralized HIV-1 subtype C Env immunogens and warrant their continued evaluation as potential components of future AIDS vaccines.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 1, 2006

Volume

352

Issue

2

Start / End Page

438 / 449

Location

United States

Related Subject Headings

  • Virology
  • Sequence Homology, Amino Acid
  • Phylogeny
  • Neutralization Tests
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Humans
  • HIV-1
  • HIV Infections
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kothe, D. L., Li, Y., Decker, J. M., Bibollet-Ruche, F., Zammit, K. P., Salazar, M. G., … Hahn, B. H. (2006). Ancestral and consensus envelope immunogens for HIV-1 subtype C. Virology, 352(2), 438–449. https://doi.org/10.1016/j.virol.2006.05.011
Kothe, Denise L., Yingying Li, Julie M. Decker, Frederic Bibollet-Ruche, Kenneth P. Zammit, Maria G. Salazar, Yalu Chen, et al. “Ancestral and consensus envelope immunogens for HIV-1 subtype C.Virology 352, no. 2 (September 1, 2006): 438–49. https://doi.org/10.1016/j.virol.2006.05.011.
Kothe DL, Li Y, Decker JM, Bibollet-Ruche F, Zammit KP, Salazar MG, et al. Ancestral and consensus envelope immunogens for HIV-1 subtype C. Virology. 2006 Sep 1;352(2):438–49.
Kothe, Denise L., et al. “Ancestral and consensus envelope immunogens for HIV-1 subtype C.Virology, vol. 352, no. 2, Sept. 2006, pp. 438–49. Pubmed, doi:10.1016/j.virol.2006.05.011.
Kothe DL, Li Y, Decker JM, Bibollet-Ruche F, Zammit KP, Salazar MG, Chen Y, Weng Z, Weaver EA, Gao F, Haynes BF, Shaw GM, Korber BTM, Hahn BH. Ancestral and consensus envelope immunogens for HIV-1 subtype C. Virology. 2006 Sep 1;352(2):438–449.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 1, 2006

Volume

352

Issue

2

Start / End Page

438 / 449

Location

United States

Related Subject Headings

  • Virology
  • Sequence Homology, Amino Acid
  • Phylogeny
  • Neutralization Tests
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Humans
  • HIV-1
  • HIV Infections