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Intranasal vaccination with the recombinant Listeria monocytogenes ΔactA prfA* mutant elicits robust systemic and pulmonary cellular responses and secretory mucosal IgA.

Publication ,  Journal Article
Qiu, J; Yan, L; Chen, J; Chen, CY; Shen, L; Letvin, NL; Haynes, BF; Freitag, N; Rong, L; Frencher, JT; Huang, D; Wang, X; Chen, ZW
Published in: Clin Vaccine Immunol
April 2011

We previously showed that recombinant (r) Listeria monocytogenes carrying ΔactA and a selected prfA* mutation (r-Listeria ΔactA prfA*) secreted >100-fold more immunogen in broth culture than wild-type r-Listeria or r-Listeria ΔactA and elicited much greater cellular and humoral immune responses than r-Listeria ΔactA after intravenous vaccination of mice. Here, we conducted comparative studies evaluating vaccine-elicited immune responses in systemic and mucosal sites after intranasal, intravenous, intraperitoneal, or subcutaneous immunization of mice with r-Listeria ΔactA prfA* vaccine candidates. Intranasal vaccination of mice with r-Listeria ΔactA prfA* vaccine candidates elicited a robust gamma interferon-positive (IFN-γ(+)) cellular response in systemic sites, although intravenous or intraperitoneal immunization was more efficient. Surprisingly, intranasal vaccination elicited an appreciable pulmonary IFN-γ(+) cellular response that was nonstatistically higher than the magnitude induced by the intravenous route but was significantly greater than that elicited by subcutaneous immunization. Furthermore, although intranasal r-Listeria ΔactA prfA* delivery induced poor systemic IgG responses, intranasal vaccination elicited appreciable secretory immunogen-specific IgA titers that were similar to or higher in mucosal fluid than those induced by subcutaneous and intravenous immunizations. Thus, intranasal vaccination with r-Listeria ΔactA prfA* appears to be a useful approach for eliciting robust systemic and pulmonary cellular responses and measurable secretory mucosal IgA titers.

Duke Scholars

Published In

Clin Vaccine Immunol

DOI

EISSN

1556-679X

Publication Date

April 2011

Volume

18

Issue

4

Start / End Page

640 / 646

Location

United States

Related Subject Headings

  • Vaccines, Attenuated
  • Vaccination
  • Peptide Termination Factors
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Membrane Proteins
  • Lung
  • Listeria monocytogenes
  • Injections, Subcutaneous
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Qiu, J., Yan, L., Chen, J., Chen, C. Y., Shen, L., Letvin, N. L., … Chen, Z. W. (2011). Intranasal vaccination with the recombinant Listeria monocytogenes ΔactA prfA* mutant elicits robust systemic and pulmonary cellular responses and secretory mucosal IgA. Clin Vaccine Immunol, 18(4), 640–646. https://doi.org/10.1128/CVI.00254-10
Qiu, Jin, Lin Yan, Jianbo Chen, Crystal Y. Chen, Ling Shen, Norman L. Letvin, Barton F. Haynes, et al. “Intranasal vaccination with the recombinant Listeria monocytogenes ΔactA prfA* mutant elicits robust systemic and pulmonary cellular responses and secretory mucosal IgA.Clin Vaccine Immunol 18, no. 4 (April 2011): 640–46. https://doi.org/10.1128/CVI.00254-10.
Qiu, Jin, et al. “Intranasal vaccination with the recombinant Listeria monocytogenes ΔactA prfA* mutant elicits robust systemic and pulmonary cellular responses and secretory mucosal IgA.Clin Vaccine Immunol, vol. 18, no. 4, Apr. 2011, pp. 640–46. Pubmed, doi:10.1128/CVI.00254-10.
Qiu J, Yan L, Chen J, Chen CY, Shen L, Letvin NL, Haynes BF, Freitag N, Rong L, Frencher JT, Huang D, Wang X, Chen ZW. Intranasal vaccination with the recombinant Listeria monocytogenes ΔactA prfA* mutant elicits robust systemic and pulmonary cellular responses and secretory mucosal IgA. Clin Vaccine Immunol. 2011 Apr;18(4):640–646.

Published In

Clin Vaccine Immunol

DOI

EISSN

1556-679X

Publication Date

April 2011

Volume

18

Issue

4

Start / End Page

640 / 646

Location

United States

Related Subject Headings

  • Vaccines, Attenuated
  • Vaccination
  • Peptide Termination Factors
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Membrane Proteins
  • Lung
  • Listeria monocytogenes
  • Injections, Subcutaneous