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Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen.

Publication ,  Journal Article
Weaver, EA; Lu, Z; Camacho, ZT; Moukdar, F; Liao, H-X; Ma, B-J; Muldoon, M; Theiler, J; Nabel, GJ; Letvin, NL; Korber, BT; Hahn, BH; Gao, F ...
Published in: J Virol
July 2006

The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide sets from heterologous subtype A, B, and C viruses. The CON6-derived vaccine was immunogenic and induced a greater number of T-cell epitope responses than any single wild-type subtype A, B, and C env immunogen and similar T-cell responses to a polyvalent vaccine. The responses were comparable to within-clade responses but significantly more than between-clade responses. The magnitude of the T-cell responses induced by CON6 (measured by individual epitope peptides) was also greater than the magnitude of responses induced by individual wild-type env immunogens. Though the limited major histocompatibility complex repertoire in inbred mice does not necessarily predict responses in nonhuman primates and humans, these results suggest that synthetic centralized env immunogens represent a promising approach for HIV-1 vaccine design that merits further characterization.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 2006

Volume

80

Issue

14

Start / End Page

6745 / 6756

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Vaccines, DNA
  • T-Lymphocytes
  • Species Specificity
  • Peptides
  • Mice, Inbred BALB C
  • Mice
  • Interferon-gamma
  • Humans
 

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Weaver, E. A., Lu, Z., Camacho, Z. T., Moukdar, F., Liao, H.-X., Ma, B.-J., … Gao, F. (2006). Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen. J Virol, 80(14), 6745–6756. https://doi.org/10.1128/JVI.02484-05
Weaver, Eric A., Zhongjing Lu, Zenaido T. Camacho, Fatiha Moukdar, Hua-Xin Liao, Ben-Jiang Ma, Mark Muldoon, et al. “Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen.J Virol 80, no. 14 (July 2006): 6745–56. https://doi.org/10.1128/JVI.02484-05.
Weaver EA, Lu Z, Camacho ZT, Moukdar F, Liao H-X, Ma B-J, et al. Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen. J Virol. 2006 Jul;80(14):6745–56.
Weaver, Eric A., et al. “Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen.J Virol, vol. 80, no. 14, July 2006, pp. 6745–56. Pubmed, doi:10.1128/JVI.02484-05.
Weaver EA, Lu Z, Camacho ZT, Moukdar F, Liao H-X, Ma B-J, Muldoon M, Theiler J, Nabel GJ, Letvin NL, Korber BT, Hahn BH, Haynes BF, Gao F. Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen. J Virol. 2006 Jul;80(14):6745–6756.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 2006

Volume

80

Issue

14

Start / End Page

6745 / 6756

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Vaccines, DNA
  • T-Lymphocytes
  • Species Specificity
  • Peptides
  • Mice, Inbred BALB C
  • Mice
  • Interferon-gamma
  • Humans