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Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis.

Publication ,  Journal Article
Yu, J-S; Peacock, JW; Vanleeuwen, S; Hsu, T; Jacobs, WR; Cayabyab, MJ; Letvin, NL; Frothingham, R; Staats, HF; Liao, H-X; Haynes, BF
Published in: Clin Vaccine Immunol
November 2006

A successful vaccine vector for human immunodeficiency virus type 1 (HIV-1) should induce anti-HIV-1 immune responses at mucosal sites. We have generated recombinant Mycobacterium smegmatis vectors that express the HIV-1 group M consensus envelope protein (Env) as a surface, intracellular, or secreted protein and have tested them in animals for induction of both anti-HIV-1 T-cell and antibody responses. Recombinant M. smegmatis engineered for expression of secreted protein induced optimal T-cell gamma interferon enzyme-linked immunospot assay responses to HIV-1 envelope in the spleen, female reproductive tract, and lungs. Unlike with the induction of T-cell responses, priming and boosting with recombinant M. smegmatis did not induce anti-HIV-1 envelope antibody responses, due primarily to insufficient protein expression of the insert. However, immunization with recombinant M. smegmatis expressing HIV-1 Env was able to prime for an HIV-1 Env protein boost for the induction of anti-HIV-1 antibody responses.

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Published In

Clin Vaccine Immunol

DOI

ISSN

1556-6811

Publication Date

November 2006

Volume

13

Issue

11

Start / End Page

1204 / 1211

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Transformation, Genetic
  • T-Lymphocytes
  • Mycobacterium smegmatis
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunity, Mucosal
  • Humans
 

Citation

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Chicago
ICMJE
MLA
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Yu, J.-S., Peacock, J. W., Vanleeuwen, S., Hsu, T., Jacobs, W. R., Cayabyab, M. J., … Haynes, B. F. (2006). Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis. Clin Vaccine Immunol, 13(11), 1204–1211. https://doi.org/10.1128/CVI.00195-06
Yu, Jae-Sung, James W. Peacock, Stacie Vanleeuwen, Tsungda Hsu, William R. Jacobs, Mark J. Cayabyab, Norman L. Letvin, et al. “Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis.Clin Vaccine Immunol 13, no. 11 (November 2006): 1204–11. https://doi.org/10.1128/CVI.00195-06.
Yu J-S, Peacock JW, Vanleeuwen S, Hsu T, Jacobs WR, Cayabyab MJ, et al. Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis. Clin Vaccine Immunol. 2006 Nov;13(11):1204–11.
Yu, Jae-Sung, et al. “Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis.Clin Vaccine Immunol, vol. 13, no. 11, Nov. 2006, pp. 1204–11. Pubmed, doi:10.1128/CVI.00195-06.
Yu J-S, Peacock JW, Vanleeuwen S, Hsu T, Jacobs WR, Cayabyab MJ, Letvin NL, Frothingham R, Staats HF, Liao H-X, Haynes BF. Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis. Clin Vaccine Immunol. 2006 Nov;13(11):1204–1211.

Published In

Clin Vaccine Immunol

DOI

ISSN

1556-6811

Publication Date

November 2006

Volume

13

Issue

11

Start / End Page

1204 / 1211

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Transformation, Genetic
  • T-Lymphocytes
  • Mycobacterium smegmatis
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunity, Mucosal
  • Humans