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Acetylation of mitochondrial proteins.

Publication ,  Journal Article
Hirschey, MD; Shimazu, T; Huang, J-Y; Verdin, E
Published in: Methods Enzymol
2009

Sirtuins (SIRT1-SIRT7) are a family of NAD(+)-dependent protein deacetylases that regulate cell survival, metabolism, and longevity. SIRT3 is localized to the mitochondria where it deacetylates several key metabolic enzymes: acetylcoenzyme A synthetase, glutamate dehydrogenase, and subunits of complex I and thereby regulates their enzymatic activity. SIRT3 is therefore emerging as a metabolic sensor that responds to change in the energy status of the cell via NAD(+) and that modulates the activity of key metabolic enzymes via protein deacetylation. Here we review experimental approaches that can be used in vitro and in vivo to study the role of acetylation in mitochondrial cell biology.

Duke Scholars

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2009

Volume

457

Start / End Page

137 / 147

Location

United States

Related Subject Headings

  • Sirtuins
  • Recombinant Proteins
  • Plasmids
  • Mitochondrial Proteins
  • Mitochondria
  • Immunoprecipitation
  • Humans
  • Biochemistry & Molecular Biology
  • Animals
  • Acetylation
 

Citation

APA
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ICMJE
MLA
NLM
Hirschey, M. D., Shimazu, T., Huang, J.-Y., & Verdin, E. (2009). Acetylation of mitochondrial proteins. Methods Enzymol, 457, 137–147. https://doi.org/10.1016/S0076-6879(09)05008-3
Hirschey, Matthew D., Tadahiro Shimazu, Jing-Yi Huang, and Eric Verdin. “Acetylation of mitochondrial proteins.Methods Enzymol 457 (2009): 137–47. https://doi.org/10.1016/S0076-6879(09)05008-3.
Hirschey MD, Shimazu T, Huang J-Y, Verdin E. Acetylation of mitochondrial proteins. Methods Enzymol. 2009;457:137–47.
Hirschey, Matthew D., et al. “Acetylation of mitochondrial proteins.Methods Enzymol, vol. 457, 2009, pp. 137–47. Pubmed, doi:10.1016/S0076-6879(09)05008-3.
Hirschey MD, Shimazu T, Huang J-Y, Verdin E. Acetylation of mitochondrial proteins. Methods Enzymol. 2009;457:137–147.

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2009

Volume

457

Start / End Page

137 / 147

Location

United States

Related Subject Headings

  • Sirtuins
  • Recombinant Proteins
  • Plasmids
  • Mitochondrial Proteins
  • Mitochondria
  • Immunoprecipitation
  • Humans
  • Biochemistry & Molecular Biology
  • Animals
  • Acetylation