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Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport.

Publication ,  Journal Article
Turi, JL; Piantadosi, CA; Stonehuerner, JD; Ghio, AJ
Published in: Biometals
October 2008

Airway epithelial cells prevent damaging effects of extracellular iron by taking up the metal and sequestering it within intracellular ferritin. Epithelial iron transport is associated with transcellular movement of other cations including changes in the expression or activity of Na, K-ATPase and epithelial Na(+) channel (ENaC). Given this relationship between iron and Na(+), we hypothesized that iron uptake by airway epithelial cells requires concurrent Na(+) transport. In preliminary studies, we found that Na(+)-free buffer blocked iron uptake by human airway epithelial cell. Na(+) channels inhibitors, including furosemide, bumetanide, and ethylisopropyl amiloride (EIPA) significantly decreased epithelial cell concentrations of non-heme iron suggesting that Na(+)-dependent iron accumulation involves generalized Na(+) flux into the cells rather than participation of one or more specific Na(+) channels. In addition, efflux of K(+) was detected during iron uptake, as was the influx of phosphate to balance the inward movement of cations. Together, these data demonstrate that intracellular iron accumulation by airway epithelium requires concurrent Na(+)/K(+)exchange.

Duke Scholars

Published In

Biometals

DOI

ISSN

0966-0844

Publication Date

October 2008

Volume

21

Issue

5

Start / End Page

571 / 580

Location

Netherlands

Related Subject Headings

  • Sodium Channels
  • Sodium Channel Blockers
  • Sodium
  • Phosphates
  • Iron
  • Ion Transport
  • Humans
  • Epithelial Cells
  • Cells, Cultured
  • Bronchi
 

Citation

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Turi, J. L., Piantadosi, C. A., Stonehuerner, J. D., & Ghio, A. J. (2008). Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport. Biometals, 21(5), 571–580. https://doi.org/10.1007/s10534-008-9143-x
Turi, Jennifer L., Claude A. Piantadosi, Jackie D. Stonehuerner, and Andrew J. Ghio. “Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport.Biometals 21, no. 5 (October 2008): 571–80. https://doi.org/10.1007/s10534-008-9143-x.
Turi JL, Piantadosi CA, Stonehuerner JD, Ghio AJ. Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport. Biometals. 2008 Oct;21(5):571–80.
Turi, Jennifer L., et al. “Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport.Biometals, vol. 21, no. 5, Oct. 2008, pp. 571–80. Pubmed, doi:10.1007/s10534-008-9143-x.
Turi JL, Piantadosi CA, Stonehuerner JD, Ghio AJ. Iron accumulation in bronchial epithelial cells is dependent on concurrent sodium transport. Biometals. 2008 Oct;21(5):571–580.
Journal cover image

Published In

Biometals

DOI

ISSN

0966-0844

Publication Date

October 2008

Volume

21

Issue

5

Start / End Page

571 / 580

Location

Netherlands

Related Subject Headings

  • Sodium Channels
  • Sodium Channel Blockers
  • Sodium
  • Phosphates
  • Iron
  • Ion Transport
  • Humans
  • Epithelial Cells
  • Cells, Cultured
  • Bronchi