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Curved FtsZ protofilaments generate bending forces on liposome membranes.

Publication ,  Journal Article
Osawa, M; Anderson, DE; Erickson, HP
Published in: EMBO J
November 18, 2009

We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force. When added to the outside of liposomes, FtsZ-YFP-mts bound and produced concave depressions, bending the membrane in the same direction as the Z ring inside liposomes. Prominent membrane tubules were then extruded at the intersections of concave depressions. We tested the effect of moving the membrane-targeting sequence (mts) from the C-terminus to the N-terminus, which is approximately 180 degrees from the C-terminal tether. When mts-FtsZ-YFP was applied to the outside of liposomes, it generated convex bulges, bending the membrane in the direction opposite to the concave depressions. We conclude that FtsZ protofilaments have a fixed direction of curvature, and the direction of membrane bending depends on which side of the bent protofilament the mts is attached to. This supports models in which the FtsZ constriction force is generated by protofilament bending.

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Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

November 18, 2009

Volume

28

Issue

22

Start / End Page

3476 / 3484

Location

England

Related Subject Headings

  • Torsion, Mechanical
  • Tensile Strength
  • Structure-Activity Relationship
  • Protein Structure, Quaternary
  • Models, Molecular
  • Models, Biological
  • Membranes
  • Liposomes
  • Developmental Biology
  • Cytoskeleton
 

Citation

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Osawa, M., Anderson, D. E., & Erickson, H. P. (2009). Curved FtsZ protofilaments generate bending forces on liposome membranes. EMBO J, 28(22), 3476–3484. https://doi.org/10.1038/emboj.2009.277
Osawa, Masaki, David E. Anderson, and Harold P. Erickson. “Curved FtsZ protofilaments generate bending forces on liposome membranes.EMBO J 28, no. 22 (November 18, 2009): 3476–84. https://doi.org/10.1038/emboj.2009.277.
Osawa M, Anderson DE, Erickson HP. Curved FtsZ protofilaments generate bending forces on liposome membranes. EMBO J. 2009 Nov 18;28(22):3476–84.
Osawa, Masaki, et al. “Curved FtsZ protofilaments generate bending forces on liposome membranes.EMBO J, vol. 28, no. 22, Nov. 2009, pp. 3476–84. Pubmed, doi:10.1038/emboj.2009.277.
Osawa M, Anderson DE, Erickson HP. Curved FtsZ protofilaments generate bending forces on liposome membranes. EMBO J. 2009 Nov 18;28(22):3476–3484.

Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

November 18, 2009

Volume

28

Issue

22

Start / End Page

3476 / 3484

Location

England

Related Subject Headings

  • Torsion, Mechanical
  • Tensile Strength
  • Structure-Activity Relationship
  • Protein Structure, Quaternary
  • Models, Molecular
  • Models, Biological
  • Membranes
  • Liposomes
  • Developmental Biology
  • Cytoskeleton