Skip to main content

Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival.

Publication ,  Journal Article
Shan, S; Robson, ND; Cao, Y; Qiao, T; Li, CY; Kontos, CD; Garcia-Blanco, M; Dewhirst, MW
Published in: FASEB J
February 2004

Neoplastic cells overexpress several angiogenic cytokines, which stimulate neovascularization. Whether the responses of the host endothelial cells to these signaling molecules affect tumor cells during early tumorigenesis has not been investigated. We investigated pre-angiogenic tumor cell survival and angiogenesis initiation by two murine tumor lines (4T1 mammary carcinoma and B16 melanoma), which constitutively expressed GFP, in dorsal skin-fold window chambers of mice treated with extracellular domain of Tie-2 (ExTek) or bFGF. ExTek reduced tumor cell survival, retarded tumor growth, and inhibited angiogenesis onset compared with controls. bFGF increased tumor cell survival and promoted earlier angiogenesis and tumor growth. Neither bFGF nor ExTek affected cell proliferation in vitro. RT-PCR showed mRNA expression of bFGF receptor 2 (FGFR2) IIIb, which does not bind bFGF efficiently, by 4T1 cells and B16 cells express FGFR1 but not FGFR2. B16 cells expressed angiopoietin (Ang) 2, but neither cell line expresses Ang1. Both tumor lines express VEGF. These findings suggested that effects of bFGF and ExTek on tumor cell survival and angiogenesis were not due to direct action but were instead a result of paracrine factors secreted by endothelial cells. These subsequent signals from endothelial cells promote early survival and proliferation of disseminated tumor cells before onset of angiogenesis.

Duke Scholars

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

February 2004

Volume

18

Issue

2

Start / End Page

326 / 328

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Solubility
  • Signal Transduction
  • Receptors, Fibroblast Growth Factor
  • Receptor, TIE-2
  • Receptor, Macrophage Colony-Stimulating Factor
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptor Protein-Tyrosine Kinases
  • RNA, Messenger
  • Peptide Fragments
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shan, S., Robson, N. D., Cao, Y., Qiao, T., Li, C. Y., Kontos, C. D., … Dewhirst, M. W. (2004). Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival. FASEB J, 18(2), 326–328. https://doi.org/10.1096/fj.03-0765fje
Shan, Siqing, Nicole D. Robson, Yiting Cao, Tong Qiao, Chuan Y. Li, Christopher D. Kontos, Mariano Garcia-Blanco, and Mark W. Dewhirst. “Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival.FASEB J 18, no. 2 (February 2004): 326–28. https://doi.org/10.1096/fj.03-0765fje.
Shan S, Robson ND, Cao Y, Qiao T, Li CY, Kontos CD, et al. Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival. FASEB J. 2004 Feb;18(2):326–8.
Shan, Siqing, et al. “Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival.FASEB J, vol. 18, no. 2, Feb. 2004, pp. 326–28. Pubmed, doi:10.1096/fj.03-0765fje.
Shan S, Robson ND, Cao Y, Qiao T, Li CY, Kontos CD, Garcia-Blanco M, Dewhirst MW. Responses of vascular endothelial cells to angiogenic signaling are important for tumor cell survival. FASEB J. 2004 Feb;18(2):326–328.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

February 2004

Volume

18

Issue

2

Start / End Page

326 / 328

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Solubility
  • Signal Transduction
  • Receptors, Fibroblast Growth Factor
  • Receptor, TIE-2
  • Receptor, Macrophage Colony-Stimulating Factor
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptor Protein-Tyrosine Kinases
  • RNA, Messenger
  • Peptide Fragments