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The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif.

Publication ,  Journal Article
Begley, CG; Aplan, PD; Denning, SM; Haynes, BF; Waldmann, TA; Kirsch, IR
Published in: Proc Natl Acad Sci U S A
December 1989

We have identified the human gene, SCL. We discovered this gene because of its involvement in a chromosomal translocation associated with the occurrence of a stem cell leukemia manifesting myeloid and lymphoid differentiation capabilities. Here we report the sequence of a cDNA for the normal SCL transcript, as well as for an aberrant fusion transcript produced in the leukemic cells. Although different at their 3' untranslated regions, both cDNAs predict a protein with primary amino acid sequence homology to the previously described amphipathic helix-loop-helix DNA binding and dimerization motif of the Ly1-1, myc, MyoD, immunoglobulin enhancer binding, daughterless, and achaete-scute families of genes. For these cDNAs, at least two different 5' ends are predicted, both of which retain this putative DNA binding domain and predict proteins in the range of 20-30 kDa. SCL mRNA is observed in "early" hematopoietic tissues. Taken together, these studies lead to the speculation that SCL plays a role in differentiation and/or commitment events during hematopoiesis.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1989

Volume

86

Issue

24

Start / End Page

10128 / 10132

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Proto-Oncogenes
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Immunoblotting
  • Humans
  • Hematopoiesis
 

Citation

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Begley, C. G., Aplan, P. D., Denning, S. M., Haynes, B. F., Waldmann, T. A., & Kirsch, I. R. (1989). The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif. Proc Natl Acad Sci U S A, 86(24), 10128–10132. https://doi.org/10.1073/pnas.86.24.10128
Begley, C. G., P. D. Aplan, S. M. Denning, B. F. Haynes, T. A. Waldmann, and I. R. Kirsch. “The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif.Proc Natl Acad Sci U S A 86, no. 24 (December 1989): 10128–32. https://doi.org/10.1073/pnas.86.24.10128.
Begley CG, Aplan PD, Denning SM, Haynes BF, Waldmann TA, Kirsch IR. The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif. Proc Natl Acad Sci U S A. 1989 Dec;86(24):10128–32.
Begley, C. G., et al. “The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif.Proc Natl Acad Sci U S A, vol. 86, no. 24, Dec. 1989, pp. 10128–32. Pubmed, doi:10.1073/pnas.86.24.10128.
Begley CG, Aplan PD, Denning SM, Haynes BF, Waldmann TA, Kirsch IR. The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif. Proc Natl Acad Sci U S A. 1989 Dec;86(24):10128–10132.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1989

Volume

86

Issue

24

Start / End Page

10128 / 10132

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Proto-Oncogenes
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Immunoblotting
  • Humans
  • Hematopoiesis