Combined chemoseparation and immunoseparation of clonogenic T lymphoma cells from human bone marrow using 2'-deoxycoformycin, deoxyadenosine, 3A1 monoclonal antibody, and complement.
Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 microM 2'-deoxycoformycin and 500 microM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-forming erythroid units, or multipotential erythroid-granulocyte-megakaryocyte-macrophage colony-forming hematopoietic progenitor cells units.
Duke Scholars
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Related Subject Headings
- Tumor Stem Cell Assay
- T-Lymphocytes
- Ribonucleosides
- Pentostatin
- Oncology & Carcinogenesis
- Lymphoma
- Humans
- Hematopoietic Stem Cells
- Deoxyadenosines
- Complement System Proteins
Citation
Published In
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Stem Cell Assay
- T-Lymphocytes
- Ribonucleosides
- Pentostatin
- Oncology & Carcinogenesis
- Lymphoma
- Humans
- Hematopoietic Stem Cells
- Deoxyadenosines
- Complement System Proteins