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Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells.

Publication ,  Journal Article
Patel, DD; Wee, SF; Whichard, LP; Bowen, MA; Pesando, JM; Aruffo, A; Haynes, BF
Published in: J Exp Med
April 1, 1995

CD6 is a 130-kD glycoprotein expressed on the surface of thymocytes and peripheral blood T cells that is involved in TCR-mediated T cell activation. In thymus, CD6 mediates interactions between thymocytes and thymic epithelial (TE) cells. In indirect immunofluorescence assays, a recombinant CD6-immunoglobulin fusion protein (CD6-Rg) bound to cultured human TE cells and to thymic fibroblasts. CD6-Rg binding to TF and TE cells was trypsin sensitive, and 54 +/- 4% of binding was divalent cation dependent. By screening the blind panel of 479 monoclonal antibodies (mAbs) from the 5th International Workshop on Human Leukocyte Differentiation Antigens for expression on human TE cells and for the ability to block CD6-Rg binding to TE cells, we found one mAb (J4-81) that significantly inhibited the binding of CD6-Rg to TE cells (60 +/- 7% inhibition). A second mAb to the surface antigen identified by mAb J4-81, J3-119, enhanced the binding of CD6-Rg to TE cells by 48 +/- 5%. Using covalent cross-linking and trypsin digestion, we found that mAb J4-81 and CD6-Rg both bound to the same 100-kD glycoprotein (CD6L-100) on the surface of TE cells. These data demonstrate that a 100-kD glycoprotein on TE cells detected by mAb J4-81 is a ligand for CD6.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

April 1, 1995

Volume

181

Issue

4

Start / End Page

1563 / 1568

Location

United States

Related Subject Headings

  • Thymus Gland
  • Recombinant Fusion Proteins
  • Organ Specificity
  • Molecular Weight
  • Immunology
  • Humans
  • Glycoproteins
  • Fibroblasts
  • Epitopes
  • Epithelium
 

Citation

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MLA
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Patel, D. D., Wee, S. F., Whichard, L. P., Bowen, M. A., Pesando, J. M., Aruffo, A., & Haynes, B. F. (1995). Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med, 181(4), 1563–1568. https://doi.org/10.1084/jem.181.4.1563
Patel, D. D., S. F. Wee, L. P. Whichard, M. A. Bowen, J. M. Pesando, A. Aruffo, and B. F. Haynes. “Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells.J Exp Med 181, no. 4 (April 1, 1995): 1563–68. https://doi.org/10.1084/jem.181.4.1563.
Patel DD, Wee SF, Whichard LP, Bowen MA, Pesando JM, Aruffo A, et al. Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med. 1995 Apr 1;181(4):1563–8.
Patel, D. D., et al. “Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells.J Exp Med, vol. 181, no. 4, Apr. 1995, pp. 1563–68. Pubmed, doi:10.1084/jem.181.4.1563.
Patel DD, Wee SF, Whichard LP, Bowen MA, Pesando JM, Aruffo A, Haynes BF. Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med. 1995 Apr 1;181(4):1563–1568.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

April 1, 1995

Volume

181

Issue

4

Start / End Page

1563 / 1568

Location

United States

Related Subject Headings

  • Thymus Gland
  • Recombinant Fusion Proteins
  • Organ Specificity
  • Molecular Weight
  • Immunology
  • Humans
  • Glycoproteins
  • Fibroblasts
  • Epitopes
  • Epithelium