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Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level.

Publication ,  Journal Article
Golightly, MG; Koren, HS; Travis, WW; Brandt, CP; Haynes, BF; Kraft, D; Rumpold, H
Published in: Cell Immunol
October 15, 1984

The monoclonal antibodies, VEP10 and OKT10, which have been shown to recognize determinants on human natural killer (NK) cells, inhibit large granular lymphocyte (LGL) NK activity against K562, MOLT4, and CEM tumor target cells in the single cell conjugate agarose assay. Inhibition of NK activity by monoclonal antibodies was expressed independently of effector-target cell binding, as inhibitory activity could be demonstrated when the monoclonal antibodies VEP10 and OKT10 were added to preformed conjugates or to the LGLs and targets prior to the binding event. In addition, this inhibition was exerted on the effector cell and not the target cell since VEP10 and OKT10 did not react with determinants on K562 target cells. Furthermore, the 4F2 monoclonal antibody, which reacted with determinants on the LGL and all of the targets used, effected no inhibition of NK activity. Inhibition of killing by OKT10 and VEP10 was specific to endogenous NK activity since the same antibodies did not inhibit antibody-dependent cellular cytotoxicity (ADCC), mixed lymphocyte-generated NK, or cytotoxic T lymphocyte (CTL) activities.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

October 15, 1984

Volume

88

Issue

2

Start / End Page

382 / 392

Location

Netherlands

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Structure-Activity Relationship
  • Killer Cells, Natural
  • Immunology
  • Humans
  • Epitopes
  • Cytotoxicity, Immunologic
  • Antigen-Antibody Complex
  • Antibodies, Monoclonal
  • 3204 Immunology
 

Citation

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Golightly, M. G., Koren, H. S., Travis, W. W., Brandt, C. P., Haynes, B. F., Kraft, D., & Rumpold, H. (1984). Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level. Cell Immunol, 88(2), 382–392. https://doi.org/10.1016/0008-8749(84)90171-0
Golightly, M. G., H. S. Koren, W. W. Travis, C. P. Brandt, B. F. Haynes, D. Kraft, and H. Rumpold. “Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level.Cell Immunol 88, no. 2 (October 15, 1984): 382–92. https://doi.org/10.1016/0008-8749(84)90171-0.
Golightly MG, Koren HS, Travis WW, Brandt CP, Haynes BF, Kraft D, et al. Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level. Cell Immunol. 1984 Oct 15;88(2):382–92.
Golightly, M. G., et al. “Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level.Cell Immunol, vol. 88, no. 2, Oct. 1984, pp. 382–92. Pubmed, doi:10.1016/0008-8749(84)90171-0.
Golightly MG, Koren HS, Travis WW, Brandt CP, Haynes BF, Kraft D, Rumpold H. Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 and VEP10 at the single cell level. Cell Immunol. 1984 Oct 15;88(2):382–392.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

October 15, 1984

Volume

88

Issue

2

Start / End Page

382 / 392

Location

Netherlands

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Structure-Activity Relationship
  • Killer Cells, Natural
  • Immunology
  • Humans
  • Epitopes
  • Cytotoxicity, Immunologic
  • Antigen-Antibody Complex
  • Antibodies, Monoclonal
  • 3204 Immunology