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Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia.

Publication ,  Journal Article
Miketova, P; Kaemingk, K; Hockenberry, M; Pasvogel, A; Hutter, J; Krull, K; Moore, IM
Published in: Biological research for nursing
January 2005

Central nervous system (CNS) treatment contributes to improved long-term disease-free survival from childhood acute lymphoblastic leukemia (ALL) by significantly decreasing the rate of disease relapse. Methotrexate (MTX), a drug commonly used for CNS treatment, has been associated with cognitive and academic problems, white-matter changes, perfusion defects, and brain atrophy. This study investigated oxidative stress as a possible mechanism of chemotherapy induced CNS injury. Unoxidized and oxidized components of phosphatidylcholine (PC), the most prevalent phospholipid in CNS cellular membranes, were measured in cerebral spinal fluid (CSF) samples obtained from 21 children diagnosed with low (n = 7), standard (n= 7), or high (n= 7) risk ALL. Children with high-risk ALL received the most MTX, especially during the most intensive phase of treatment (consolidation). Phospholipids were extracted from CSF samples obtained at diagnosis and during the induction, consolidation, and continuation treatment phases. Unoxidized and oxidized PC were measured by normal phase high-performance liquid chromatography at 2 ultraviolet wavelengths (206 and 234 nm, respectively). Data were analyzed by 2-way repeated-measures analysis of variance. Results support the hypotheses that the highest levels of oxidized PC would be observed during the most intensive phase of ALL therapy and in the high-risk ALL group. Findings provide preliminary evidence for chemotherapy-induced oxidative stress in CNS membrane phospholipids.

Duke Scholars

Published In

Biological research for nursing

DOI

EISSN

1552-4175

ISSN

1099-8004

Publication Date

January 2005

Volume

6

Issue

3

Start / End Page

187 / 195

Related Subject Headings

  • Precursor Cell Lymphoblastic Leukemia-Lymphoma
  • Phosphatidylcholines
  • Oxidative Stress
  • Nursing
  • Methotrexate
  • Male
  • Lipid Peroxidation
  • Injections, Spinal
  • Humans
  • Female
 

Citation

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Miketova, P., Kaemingk, K., Hockenberry, M., Pasvogel, A., Hutter, J., Krull, K., & Moore, I. M. (2005). Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia. Biological Research for Nursing, 6(3), 187–195. https://doi.org/10.1177/1099800404271916
Miketova, Petra, Kris Kaemingk, Marilyn Hockenberry, Alice Pasvogel, John Hutter, Kevin Krull, and Ida M. Moore. “Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia.Biological Research for Nursing 6, no. 3 (January 2005): 187–95. https://doi.org/10.1177/1099800404271916.
Miketova P, Kaemingk K, Hockenberry M, Pasvogel A, Hutter J, Krull K, et al. Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia. Biological research for nursing. 2005 Jan;6(3):187–95.
Miketova, Petra, et al. “Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia.Biological Research for Nursing, vol. 6, no. 3, Jan. 2005, pp. 187–95. Epmc, doi:10.1177/1099800404271916.
Miketova P, Kaemingk K, Hockenberry M, Pasvogel A, Hutter J, Krull K, Moore IM. Oxidative changes in cerebral spinal fluid phosphatidylcholine during treatment for acute lymphoblastic leukemia. Biological research for nursing. 2005 Jan;6(3):187–195.
Journal cover image

Published In

Biological research for nursing

DOI

EISSN

1552-4175

ISSN

1099-8004

Publication Date

January 2005

Volume

6

Issue

3

Start / End Page

187 / 195

Related Subject Headings

  • Precursor Cell Lymphoblastic Leukemia-Lymphoma
  • Phosphatidylcholines
  • Oxidative Stress
  • Nursing
  • Methotrexate
  • Male
  • Lipid Peroxidation
  • Injections, Spinal
  • Humans
  • Female