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Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers.

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Wang, S; Kennedy, JS; West, K; Montefiori, DC; Coley, S; Lawrence, J; Shen, S; Green, S; Rothman, AL; Ennis, FA; Arthos, J; Pal, R; Markham, P; Lu, S
Published in: Vaccine
July 23, 2008

An optimally effective AIDS vaccine would likely require the induction of both neutralizing antibody and cell-mediated immune responses, which has proven difficult to obtain in previous clinical trials. Here we report on the induction of human immunodeficiency virus type-1 (HIV-1)-specific immune responses in healthy adult volunteers that received the multi-gene, polyvalent, DNA prime-protein boost HIV-1 vaccine formulation, DP6-001, in a Phase I clinical trial. Robust cross-subtype HIV-1 specific T cell responses were detected in IFN-gamma ELISPOT assays. Furthermore, we detected high titer serum antibody responses that recognized a wide range of primary HIV-1 Env antigens and also neutralized pseudotyped viruses that express the primary Env antigens from multiple HIV-1 subtypes. These findings demonstrate that the DNA prime-protein boost approach is an effective immunization method to elicit both humoral and cell-mediated immune responses in humans, and that a polyvalent Env formulation could generate broad immune responses against HIV-1 viruses with diverse genetic backgrounds.

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Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

July 23, 2008

Volume

26

Issue

31

Start / End Page

3947 / 3957

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Subunit
  • Vaccines, DNA
  • T-Lymphocytes
  • Neutralization Tests
  • Middle Aged
  • Male
  • Interferon-gamma
  • Immunization, Secondary
  • Humans
 

Citation

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Wang, S., Kennedy, J. S., West, K., Montefiori, D. C., Coley, S., Lawrence, J., … Lu, S. (2008). Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers. Vaccine. Netherlands. https://doi.org/10.1016/j.vaccine.2007.12.060
Wang, Shixia, Jeffrey S. Kennedy, Kim West, David C. Montefiori, Scott Coley, John Lawrence, Siyuan Shen, et al. “Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers.Vaccine, July 23, 2008. https://doi.org/10.1016/j.vaccine.2007.12.060.
Wang S, Kennedy JS, West K, Montefiori DC, Coley S, Lawrence J, et al. Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers. Vol. 26, Vaccine. 2008. p. 3947–57.
Wang, Shixia, et al. “Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers.Vaccine, vol. 26, no. 31, 23 July 2008, pp. 3947–57. Pubmed, doi:10.1016/j.vaccine.2007.12.060.
Wang S, Kennedy JS, West K, Montefiori DC, Coley S, Lawrence J, Shen S, Green S, Rothman AL, Ennis FA, Arthos J, Pal R, Markham P, Lu S. Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers. Vaccine. 2008. p. 3947–3957.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

July 23, 2008

Volume

26

Issue

31

Start / End Page

3947 / 3957

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, Subunit
  • Vaccines, DNA
  • T-Lymphocytes
  • Neutralization Tests
  • Middle Aged
  • Male
  • Interferon-gamma
  • Immunization, Secondary
  • Humans