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Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques.

Publication ,  Journal Article
Rasmussen, RA; Hofmann-Lehmann, R; Li, P-L; Vlasak, J; Schmitz, JE; Reimann, KA; Kuroda, MJ; Letvin, NL; Montefiori, DC; McClure, HM; Ruprecht, RM
Published in: AIDS
April 12, 2002

OBJECTIVE: To directly examine the role of CD8+ T cells in controlling viremia and disease during chronic, low-level primate immunodeficiency virus infection in DNA prime/protein boost-vaccinated macaques. BACKGROUND: A cohort of macaques, vaccinated with either a DNA prime/HIV-1 gp160 boost regimen or with gp160 alone was previously protected partially from sequential challenges with non-pathogenic and pathogenic strains of chimeric simian/human immunodeficiency virus (SHIV). In this study, the effect of temporary ablation of CD8+ T cells in these animals was examined. METHODS: Animals were treated with an anti-CD8 antibody and CD8+ T-cell levels in peripheral blood, plasma viral loads, peripheral blood mononuclear cell-associated virus levels, neutralizing antibody (nAb) titers and simian immunodeficiency virus Gag-specific CD8+ T-cell numbers were followed. RESULTS: Plasma viremia rose sharply in direct synchrony with a rapid but transient drop in CD8+ T cells. However, although levels of cell-associated virus also rose concomitantly, peak levels were much lower than those in virus-challenged, naive animals. In addition, despite a rise of pathogenic SHIV89.6P RNA levels in three animals, CD4+ T-cell counts remained unchanged. In each of these animals, neutralizing antibody titers against the pathogenic SHIV89.6P strain were high. CONCLUSIONS: The results indicate that CD8+ T cells play a key role in suppressing viremia in a chronically infected host. In addition, the results suggest that in the absence of CD8+ T cells, nAb may act as an effective second line of defense by limiting both the spread of infectious virus to new target cells and CD4+ T-cell loss.

Duke Scholars

Published In

AIDS

DOI

ISSN

0269-9370

Publication Date

April 12, 2002

Volume

16

Issue

6

Start / End Page

829 / 838

Location

England

Related Subject Headings

  • Virology
  • Viremia
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Neutralization Tests
  • Macaca mulatta
  • Lymphocyte Depletion
  • HIV Infections
  • HIV
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
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Rasmussen, R. A., Hofmann-Lehmann, R., Li, P.-L., Vlasak, J., Schmitz, J. E., Reimann, K. A., … Ruprecht, R. M. (2002). Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques. AIDS, 16(6), 829–838. https://doi.org/10.1097/00002030-200204120-00002
Rasmussen, Robert A., Regina Hofmann-Lehmann, Pei-Lin Li, Josef Vlasak, Joern E. Schmitz, Keith A. Reimann, Marcelo J. Kuroda, et al. “Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques.AIDS 16, no. 6 (April 12, 2002): 829–38. https://doi.org/10.1097/00002030-200204120-00002.
Rasmussen RA, Hofmann-Lehmann R, Li P-L, Vlasak J, Schmitz JE, Reimann KA, et al. Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques. AIDS. 2002 Apr 12;16(6):829–38.
Rasmussen, Robert A., et al. “Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques.AIDS, vol. 16, no. 6, Apr. 2002, pp. 829–38. Pubmed, doi:10.1097/00002030-200204120-00002.
Rasmussen RA, Hofmann-Lehmann R, Li P-L, Vlasak J, Schmitz JE, Reimann KA, Kuroda MJ, Letvin NL, Montefiori DC, McClure HM, Ruprecht RM. Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques. AIDS. 2002 Apr 12;16(6):829–838.

Published In

AIDS

DOI

ISSN

0269-9370

Publication Date

April 12, 2002

Volume

16

Issue

6

Start / End Page

829 / 838

Location

England

Related Subject Headings

  • Virology
  • Viremia
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Neutralization Tests
  • Macaca mulatta
  • Lymphocyte Depletion
  • HIV Infections
  • HIV
  • Disease Models, Animal