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Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein.

Publication ,  Journal Article
Gao, F; Weaver, EA; Lu, Z; Li, Y; Liao, H-X; Ma, B; Alam, SM; Scearce, RM; Sutherland, LL; Yu, J-S; Decker, JM; Shaw, GM; Montefiori, DC ...
Published in: J Virol
January 2005

Genetic variation of human immunodeficiency virus (HIV-1) represents a major obstacle for AIDS vaccine development. To decrease the genetic distances between candidate immunogens and field virus strains, we have designed and synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary HIV-1 subtypes and recombinants. This novel envelope gene expresses a glycoprotein that binds soluble CD4, utilizes CCR5 but not CXCR4 as a coreceptor, and mediates HIV-1 entry. Key linear, conformational, and glycan-dependent monoclonal antibody epitopes are preserved in CON6, and the glycoprotein is recognized equally well by sera from individuals infected with different HIV-1 subtypes. When used as a DNA vaccine followed by a recombinant vaccinia virus boost in BALB/c mice, CON6 env gp120 and gp140CF elicited gamma interferon-producing T-cell responses that recognized epitopes within overlapping peptide pools from three HIV-1 Env proteins, CON6, MN (subtype B), and Chn19 (subtype C). Sera from guinea pigs immunized with recombinant CON6 Env gp120 and gp140CF glycoproteins weakly neutralized selected HIV-1 primary isolates. Thus, the computer-generated "consensus" env genes are capable of expressing envelope glycoproteins that retain the structural, functional, and immunogenic properties of wild-type HIV-1 envelopes.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 2005

Volume

79

Issue

2

Start / End Page

1154 / 1163

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • T-Lymphocytes
  • Receptors, CCR5
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Humans
  • HIV-1
  • HIV Envelope Protein gp160
 

Citation

APA
Chicago
ICMJE
MLA
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Gao, F., Weaver, E. A., Lu, Z., Li, Y., Liao, H.-X., Ma, B., … Haynes, B. F. (2005). Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein. J Virol, 79(2), 1154–1163. https://doi.org/10.1128/JVI.79.2.1154-1163.2005
Gao, Feng, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S Munir Alam, et al. “Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein.J Virol 79, no. 2 (January 2005): 1154–63. https://doi.org/10.1128/JVI.79.2.1154-1163.2005.
Gao F, Weaver EA, Lu Z, Li Y, Liao H-X, Ma B, et al. Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein. J Virol. 2005 Jan;79(2):1154–63.
Gao, Feng, et al. “Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein.J Virol, vol. 79, no. 2, Jan. 2005, pp. 1154–63. Pubmed, doi:10.1128/JVI.79.2.1154-1163.2005.
Gao F, Weaver EA, Lu Z, Li Y, Liao H-X, Ma B, Alam SM, Scearce RM, Sutherland LL, Yu J-S, Decker JM, Shaw GM, Montefiori DC, Korber BT, Hahn BH, Haynes BF. Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein. J Virol. 2005 Jan;79(2):1154–1163.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 2005

Volume

79

Issue

2

Start / End Page

1154 / 1163

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • T-Lymphocytes
  • Receptors, CCR5
  • Molecular Sequence Data
  • Mice, Inbred BALB C
  • Mice
  • Humans
  • HIV-1
  • HIV Envelope Protein gp160