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Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.

Publication ,  Journal Article
Li, M; Gao, F; Mascola, JR; Stamatatos, L; Polonis, VR; Koutsoukos, M; Voss, G; Goepfert, P; Gilbert, P; Greene, KM; Bilska, M; Kothe, DL ...
Published in: J Virol
August 2005

Induction of broadly cross-reactive neutralizing antibodies is a high priority for AIDS vaccine development but one that has proven difficult to be achieved. While most immunogens generate antibodies that neutralize a subset of T-cell-line-adapted strains of human immunodeficiency virus type 1 (HIV-1), none so far have generated a potent, broadly cross-reactive response against primary isolates of the virus. Even small increments in immunogen improvement leading to increases in neutralizing antibody titers and cross-neutralizing activity would accelerate vaccine development; however, a lack of uniformity in target strains used by different investigators to assess cross-neutralization has made the comparison of vaccine-induced antibody responses difficult. Thus, there is an urgent need to establish standard panels of HIV-1 reference strains for wide distribution. To facilitate this, full-length gp160 genes were cloned from acute and early subtype B infections and characterized for use as reference reagents to assess neutralizing antibodies against clade B HIV-1. Individual gp160 clones were screened for infectivity as Env-pseudotyped viruses in a luciferase reporter gene assay in JC53-BL (TZM-bl) cells. Functional env clones were sequenced and their neutralization phenotypes characterized by using soluble CD4, monoclonal antibodies, and serum samples from infected individuals and noninfected recipients of a recombinant gp120 vaccine. Env clones from 12 R5 primary HIV-1 isolates were selected that were not unusually sensitive or resistant to neutralization and comprised a wide spectrum of genetic, antigenic, and geographic diversity. These reference reagents will facilitate proficiency testing and other validation efforts aimed at improving assay performance across laboratories and can be used for standardized assessments of vaccine-elicited neutralizing antibodies.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

August 2005

Volume

79

Issue

16

Start / End Page

10108 / 10125

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Neutralization Tests
  • Molecular Sequence Data
  • Humans
  • HIV-1
  • HIV Antibodies
  • Genes, env
  • Gene Products, env
  • Cloning, Molecular
 

Citation

APA
Chicago
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MLA
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Li, M., Gao, F., Mascola, J. R., Stamatatos, L., Polonis, V. R., Koutsoukos, M., … Montefiori, D. C. (2005). Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol, 79(16), 10108–10125. https://doi.org/10.1128/JVI.79.16.10108-10125.2005
Li, Ming, Feng Gao, John R. Mascola, Leonidas Stamatatos, Victoria R. Polonis, Marguerite Koutsoukos, Gerald Voss, et al. “Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.J Virol 79, no. 16 (August 2005): 10108–25. https://doi.org/10.1128/JVI.79.16.10108-10125.2005.
Li M, Gao F, Mascola JR, Stamatatos L, Polonis VR, Koutsoukos M, et al. Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol. 2005 Aug;79(16):10108–25.
Li, Ming, et al. “Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.J Virol, vol. 79, no. 16, Aug. 2005, pp. 10108–25. Pubmed, doi:10.1128/JVI.79.16.10108-10125.2005.
Li M, Gao F, Mascola JR, Stamatatos L, Polonis VR, Koutsoukos M, Voss G, Goepfert P, Gilbert P, Greene KM, Bilska M, Kothe DL, Salazar-Gonzalez JF, Wei X, Decker JM, Hahn BH, Montefiori DC. Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol. 2005 Aug;79(16):10108–10125.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

August 2005

Volume

79

Issue

16

Start / End Page

10108 / 10125

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, Synthetic
  • Neutralization Tests
  • Molecular Sequence Data
  • Humans
  • HIV-1
  • HIV Antibodies
  • Genes, env
  • Gene Products, env
  • Cloning, Molecular