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Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model.

Publication ,  Journal Article
Earl, PL; Wyatt, LS; Montefiori, DC; Bilska, M; Woodward, R; Markham, PD; Malley, JD; Vogel, TU; Allen, TM; Watkins, DI; Miller, N; Moss, B
Published in: Virology
March 15, 2002

Rhesus macaques were immunized with a replication-deficient vaccinia virus (MVA) expressing human immunodeficiency virus type 1 89.6 envelope (env) and SIV gagpol (MVA/SHIV89.6) with or without a protein boost consisting of soluble 89.6 env (gp140). Immunization with MVA/SHIV89.6 alone elicited binding antibodies in all animals and neutralizing antibodies in 5 of 15 animals. Both types of antibodies were enhanced by protein boosting. In addition, CD8 cells exhibiting CM9 tetramer binding were detected in the subset of animals that were Mamu-A*01 positive. Animals were challenged intravenously with either SHIV-89.6 (Study 1) or the more pathogenic derivative SHIV-89.6P (Study 2). In Study 1, all control and vaccinated animals except one became infected. However, the levels of viremia were as follows: controls > rMVA alone > rMVA + protein. The differences were statistically significant between immunized and control groups but not between the two immunized groups. In Study 2, all animals became infected; however, the vaccinated group exhibited a 5-fold reduction in peak viremia and a 10-fold reduction in the postacute phase viremia in comparison to the controls. All of the controls required euthanasia by 10 months after challenge. A relationship between vaccine-induced antibody titers and reduction in virus burden was observed in both studies. Thus, immunization with MVA/SHIV89.6 alone or with a protein boost stimulated both arms of the immune system and resulted in significant control of viremia and delayed progression to disease after challenge with SHIV-89.6P.

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Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 15, 2002

Volume

294

Issue

2

Start / End Page

270 / 281

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Viral Load
  • Vaccines, DNA
  • T-Lymphocytes, Cytotoxic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Recombinant Fusion Proteins
  • Oligopeptides
  • Macaca mulatta
 

Citation

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Earl, P. L., Wyatt, L. S., Montefiori, D. C., Bilska, M., Woodward, R., Markham, P. D., … Moss, B. (2002). Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model. Virology, 294(2), 270–281. https://doi.org/10.1006/viro.2001.1345
Earl, Patricia L., Linda S. Wyatt, David C. Montefiori, Miroslawa Bilska, Ruth Woodward, Phillip D. Markham, James D. Malley, et al. “Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model.Virology 294, no. 2 (March 15, 2002): 270–81. https://doi.org/10.1006/viro.2001.1345.
Earl PL, Wyatt LS, Montefiori DC, Bilska M, Woodward R, Markham PD, et al. Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model. Virology. 2002 Mar 15;294(2):270–81.
Earl, Patricia L., et al. “Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model.Virology, vol. 294, no. 2, Mar. 2002, pp. 270–81. Pubmed, doi:10.1006/viro.2001.1345.
Earl PL, Wyatt LS, Montefiori DC, Bilska M, Woodward R, Markham PD, Malley JD, Vogel TU, Allen TM, Watkins DI, Miller N, Moss B. Comparison of vaccine strategies using recombinant env-gag-pol MVA with or without an oligomeric Env protein boost in the SHIV rhesus macaque model. Virology. 2002 Mar 15;294(2):270–281.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 15, 2002

Volume

294

Issue

2

Start / End Page

270 / 281

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Viral Load
  • Vaccines, DNA
  • T-Lymphocytes, Cytotoxic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Recombinant Fusion Proteins
  • Oligopeptides
  • Macaca mulatta