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DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge.

Publication ,  Journal Article
Rosati, M; von Gegerfelt, A; Roth, P; Alicea, C; Valentin, A; Robert-Guroff, M; Venzon, D; Montefiori, DC; Markham, P; Felber, BK; Pavlakis, GN
Published in: J Virol
July 2005

We have tested the efficacy of DNA immunization as a single vaccination modality for rhesus macaques followed by highly pathogenic SIVmac251 challenge. To further improve immunogenicity of the native proteins, we generated expression vectors producing fusion of the proteins Gag and Env to the secreted chemokine MCP3, targeting the viral proteins to the secretory pathway and to a beta-catenin (CATE) peptide, targeting the viral proteins to the intracellular degradation pathway. Macaques immunized with vectors expressing the MCP3-tagged fusion proteins developed stronger antibody responses. Following mucosal challenge with pathogenic SIVmac251, the vaccinated animals showed a statistically significant decrease in viral load (P = 0.010). Interestingly, macaques immunized with a combination of vectors expressing three forms of antigens (native protein and MCP3 and CATE fusion proteins) showed the strongest decrease in viral load (P = 0.0059). Postchallenge enzyme-linked immunospot values for Gag and Env as well as gag-specific T-helper responses correlated with control of viremia. Our data show that the combinations of DNA vaccines producing native and modified forms of antigens elicit more balanced immune responses able to significantly reduce viremia for a long period (8 months) following pathogenic challenge with SIVmac251.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 2005

Volume

79

Issue

13

Start / End Page

8480 / 8492

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Vaccines, DNA
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Macaca mulatta
  • Lymphocyte Activation
  • Humans
  • HIV Antigens
  • Cell Line
 

Citation

APA
Chicago
ICMJE
MLA
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Rosati, M., von Gegerfelt, A., Roth, P., Alicea, C., Valentin, A., Robert-Guroff, M., … Pavlakis, G. N. (2005). DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge. J Virol, 79(13), 8480–8492. https://doi.org/10.1128/JVI.79.13.8480-8492.2005
Rosati, Margherita, Agneta von Gegerfelt, Patricia Roth, Candido Alicea, Antonio Valentin, Marjorie Robert-Guroff, David Venzon, et al. “DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge.J Virol 79, no. 13 (July 2005): 8480–92. https://doi.org/10.1128/JVI.79.13.8480-8492.2005.
Rosati M, von Gegerfelt A, Roth P, Alicea C, Valentin A, Robert-Guroff M, et al. DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge. J Virol. 2005 Jul;79(13):8480–92.
Rosati, Margherita, et al. “DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge.J Virol, vol. 79, no. 13, July 2005, pp. 8480–92. Pubmed, doi:10.1128/JVI.79.13.8480-8492.2005.
Rosati M, von Gegerfelt A, Roth P, Alicea C, Valentin A, Robert-Guroff M, Venzon D, Montefiori DC, Markham P, Felber BK, Pavlakis GN. DNA vaccines expressing different forms of simian immunodeficiency virus antigens decrease viremia upon SIVmac251 challenge. J Virol. 2005 Jul;79(13):8480–8492.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 2005

Volume

79

Issue

13

Start / End Page

8480 / 8492

Location

United States

Related Subject Headings

  • Virology
  • Viremia
  • Vaccines, DNA
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Macaca mulatta
  • Lymphocyte Activation
  • Humans
  • HIV Antigens
  • Cell Line